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Invitrogen
ATP7A Monoclonal Antibody (L60/4), PE
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图: 1 / 3
ATP7A Antibody (MA5-45521) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using NIH 3T3 (NIH 3T3). Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Copper Transporting ATPase 1 monoclonal antibody (Product # MA5-45521) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum, Cytoplasm, Golgi Apparatus, Trans-Golgi Network Membrane, ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-45521
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
L60/4
抗原
Synthetic peptide amino acids 42-61 (cytoplasmic C-terminus) of human Copper- transporting ATPase1
偶联物
PE
PE
PE
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein G
保存液
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
内含物
no preservative
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
1 µg/mL of MA5-45521 was sufficient for detection of Copper-transporting ATPase1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.|Detects approximately 180kDa in rat brain membrane preparations.
This antibody was formerly sold as clone S60-4.
靶标信息
ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-translocating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ATP 7A; Copper pump 1; Copper-transporting ATPase 1; FLJ17790; MC 1; Menke; Menkes disease-associated protein; Menkes disease-associated protein homolog; Menkes protein; Menkes syndrome; OHS; OTTHUMP00000062077
基因别名: ATP7A; MC1; MNK
UniProt ID: (Mouse) Q64430, (Rat) P70705
Entrez Gene ID: (Mouse) 11977, (Rat) 24941
nucleotide binding
copper-exporting ATPase activity
copper ion transmembrane transporter activity
copper ion binding
protein binding
ATP binding
superoxide dismutase copper chaperone activity
hydrolase activity
cation-transporting ATPase activity
copper-dependent protein binding
metal ion binding
molecular_function
primary active transporter
blood vessel development
release of cytochrome c from mitochondria
blood vessel remodeling
regulation of oxidative phosphorylation
tryptophan metabolic process
tyrosine metabolic process
catecholamine metabolic process
transport
ion transport
cation transport
copper ion transport
cellular copper ion homeostasis
mitochondrion organization
locomotory behavior
detoxification of copper ion
regulation of gene expression
copper ion import
plasma membrane copper ion transport
peptidyl-lysine modification
removal of superoxide radicals
cerebellar Purkinje cell differentiation
pyramidal neuron development
central nervous system neuron development
metal ion transport
extracellular matrix organization
collagen fibril organization
hair follicle morphogenesis
hindlimb morphogenesis
T-helper cell differentiation
epinephrine metabolic process
norepinephrine metabolic process
dopamine metabolic process
norepinephrine biosynthetic process
serotonin metabolic process
positive regulation of catalytic activity
negative regulation of catalytic activity
pigmentation
negative regulation of neuron apoptotic process
skin development
ATP metabolic process
response to copper ion
elastic fiber assembly
lung alveolus development
neuron projection morphogenesis
dendrite morphogenesis
cartilage development
positive regulation of oxidoreductase activity
elastin biosynthetic process
copper ion export
in utero embryonic development
lactation
response to iron(III) ion
response to zinc ion
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