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Invitrogen
Ankyrin 1 Monoclonal Antibody (N388A/10), PerCP
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FIGURE: 1 / 3
Ankyrin 1 Antibody (MA5-45731) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Ankyrin R monoclonal antibody (Product # MA5-45731) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000; 1:5,000 fo... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45731
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
N388A/10
Immunogen
Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R
Conjugate
PerCP
PerCP
PerCP
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:100 dilution of MA5-45731 was sufficient for detection of Ankyrin R in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 200kDa. Does not cross-react with Ankyrin-B or Ankyrin-G.
This antibody was formerly sold as clone S388A-10.
Target Information
Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ANK-1; ankyrin 1, erythroid; Ankyrin-1; Ankyrin-R; Erythrocyte ankyrin; normoblastic anemia; rCG_43073
Gene Aliases: ANK; Ank-1; ANK1; nb; pale
UniProt ID: (Mouse) Q02357
Entrez Gene ID: (Mouse) 11733, (Rat) 306570
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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