Invitrogen

MARVELD2 Polyclonal Antibody

Name: MARVELD2 Polyclonal Antibody
Brand: Invitrogen
SKU: 48-8400

20个已发表图片

29 篇参考文献

查看其他6个MARVELD2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (5)
已发表图片 (20)

MARVELD2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 25

MARVELD2 Antibody (48-8400) in ICC/IF

Immunofluorescence analysis of Marveld2/ Tricellulin was performed using 90% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MARVELD2/Tricellulin Rabbit Polyclonal Antibody (Product # 48-8400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fl... View More

MARVELD2 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

MARVELD2 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

MARVELD2 Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF)

MARVELD2 Antibody in Immunohistochemistry (IHC)

MARVELD2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

Immunogen image

Immunogen Image MARVELD2 Polyclonal Antibody

产品信息

48-8400

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-3 µg/mL

查看16篇已发表文章 16篇已发表文章

免疫组化 (IHC)

查看4篇已发表文章 4篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

2-4 µg/mL

查看7篇已发表文章 7篇已发表文章

流式细胞分析 (Flow)

3-5 µg/1x10^6 cells

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

C. elegans, Dog, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein derived from the C-terminal region of the human Tricellulin protein (Accession# NP_001033692, Q8N4S9), which is identical to rhesus monkey and chimpanzee sequences, and 89% homologous to rat and mouse, 88% homologous to bovine and canine, and 86% homologous to horse sequence

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2533851

产品详细信息

This antibody is specific for the Tricellulin/MARVELD-2 protein. On western blots, it identifies the target band at ~64 kDa. Reactivity has been confirmed with human Caco-2, dog MDCK, mouse IMCD-3 cells, and mouse kidney lysates by western blotting, and with mouse MTE7b cells by immunocytochemistry. Based on amino acid sequence homology, reactivity with Rhesus monkey, chimpanzee, rat, bovine, and horse is expected.

靶标信息

Tight junctions form an important barrier of paracellular transport in epithelial cells. Sealing of two adjacent cells at bicellular tight junctions, a point where three adjacent cells are in contact with each other. Tricellulin is the first protein identified that specifically concentrates in tricellular tight junctions. This protein has four membrane spanning domains, similarly to claudins. Tricellulin expression is high in epithelium-derived tissues, such as small intestine, kidney and lung. Functional evidence for the role of tricellulin in tight junction formation comes from siRNA studies, where suppression of its expression leads to compromised epithelial barrier and tight junction formation. Loss of function in tricellulin mutants missing all or most of a conserved region in the cytosolic domain which binds to the cytosolic scaffolding protein ZO-1, indicate that interaction with other known tight junction proteins plays an important role for the function of tricellulin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。