Invitrogen

MASH1 Monoclonal Antibody (24B72D11), eBioscience™

4个已发表图片
6 篇参考文献
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说明书
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产品信息

14-5794-82

应用
建议稀释比
已发表文章

免疫印迹 (WB)

5 µg/mL
查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

-
查看4篇已发表文章 4篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-Dependent
-

免疫细胞化学 (ICC/IF)

20 µg/mL
-
产品规格

种属反应

Mouse, Rat

已发表种属

C. elegans, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

24B72D11

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2572887

产品详细信息

Description: The monoclonal antibody 24B72D11 recognizes mouse and rat Mammalian achaete-scute homolog 1 (MASH1), a 34 kDa basic helix-loop-helix (bHLH) transcription factor. MASH1, like family members Olig2 and Ngn2, is expressed in progenitor cells throughout the nervous system and retina and is a key regulator of cell fate specification. Expression of MASH1 is regulated by the Delta/Notch1 pathway through direct binding of Hes1 to MASH1 as well as competitive binding to MASH1 hetero-dimerizing proteins. At a transcriptional level, MASH1 expression is controlled by notch-mediated regulation of sox2, which binds to the MASH1 promoter region up-regulating expression. MASH1 has been implicated in the formation of multiple neural cell types including dopaminergic, serotonergic, glutaminergic and GABAergic neurons. Overexpression of MASH1 has been shown in small cell lung carcinoma and medullary thyroid cancer.

Applications Reported: This 24B72D11 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, and immunocytochemistry.

Applications Tested: This 24B72D11 antibody has been tested by western blot on reduced PC12 cells and can be used at less than or equal to 5 µg/mL. The 24B72D11 antibody has also been tested by immunocytochemistry on fixed and permeablized PC12 cells at can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

ASCL1 (also known as ASH1) is a basic helix-loop-helix transcription factor that is required for early development of the nervous system. Expressed in fetal brain, ASCL1 is essential for the proper development of autonomic neurons and for the survival of subsets of autonomic neurons. ASCL1 interaction with MEF-2A may regulate the expression of specific genes that are critical for the formation of distinct neuronal circuits within the central nervous system. The high level of ASCL1 expression in neuroendocrine tumors, such as medullary thyroid cancer, small cell lung cancer and lung cancer with neuroendocrine features may provide a useful marker for cancers with neuroendocrine features. Mapping to human chromosome 12, the ASCL1 gene contains a trinucleotide repeat region, making this locus a candidate for inherited disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: achaete-scute complex homolog 1; achaete-scute complex homolog-like 1; achaete-scute complex-like protein 1; Achaete-scute homolog 1; ASH-1; KMT2H; mammalian achaete scute homolog 1; mASH-1

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基因别名: AI225900; Ascl1; ASH1; bHLHa46; Mash-1; Mash1

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UniProt ID: (Rat) P19359, (Mouse) Q02067

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Entrez Gene ID: (Rat) 64186, (Mouse) 17172

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功能
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, transcription factor binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein homodimerization activity bHLH transcription factor binding sequence-specific DNA binding E-box binding protein binding protein dimerization activity basic helix-loop-helix transcription factor
参与通路
negative regulation of transcription from RNA polymerase II promoter neuron migration noradrenergic neuron development noradrenergic neuron fate commitment transcription, DNA-templated regulation of transcription from RNA polymerase II promoter Notch signaling pathway regulation of mitotic cell cycle pattern specification process nervous system development neuroblast fate determination neuroblast proliferation regulation of Notch signaling pathway glial cell differentiation response to lithium ion regulation of gene expression oligodendrocyte development spinal cord association neuron differentiation spinal cord oligodendrocyte cell differentiation spinal cord oligodendrocyte cell fate specification vestibular nucleus development oligodendrocyte cell fate commitment forebrain neuron differentiation cerebral cortex GABAergic interneuron differentiation commitment of neuronal cell to specific neuron type in forebrain central nervous system neuron development cerebral cortex development neurogenesis cell differentiation neuron differentiation regulation of epithelial cell differentiation response to retinoic acid regulation of cell proliferation negative regulation of apoptotic process positive regulation of neuron apoptotic process negative regulation of neuron differentiation positive regulation of neuron differentiation positive regulation of Notch signaling pathway positive regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell maturation neuron fate commitment neuron fate specification neuron development generation of neurons oligodendrocyte differentiation regulation of neurogenesis positive regulation of neurogenesis musculoskeletal movement, spinal reflex action response to folic acid subpallium neuron fate commitment regulation of timing of subpallium neuron differentiation olfactory pit development ventral spinal cord interneuron fate commitment lung neuroendocrine cell differentiation stomach neuroendocrine cell differentiation carotid body glomus cell differentiation adrenal chromaffin cell differentiation sympathetic ganglion development response to epidermal growth factor cellular response to magnetism positive regulation of neural precursor cell proliferation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development enteric nervous system development sympathetic nervous system development parasympathetic nervous system development
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