Invitrogen

MAVS Monoclonal Antibody (ABM28H9), PE, eBioscience™

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他22个MAVS抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (1)
高级验证 (1)

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MAVS Antibody (12-9835-42) in Flow

HEL cells were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with Mouse IgG2b kappa Isotype Control, PE (Product # 12-4732-82) (blue histogram) or MAVS Monoclonal Antibody, PE (purple histogram). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).

Product Image Gallery

MAVS Antibody — Staining of A549 cell line transfected with either negative control siRNA (purple histogram) or MAVS gene siRNA (blue histogram). As expected, clone ABM28H9 stains A549 cells transfected with negative control siRNA brighter than cells transfected with MAVS-specific siRNA. Details: A549 cells were transfected with either Silencer Select Negative Control No. 1 siRNA (purple) or with MAVS gene Silencer Select siRNA (blue) using Neon Transfection System. The cells were then cultured for 3 days and stained with Fixable Viability Dye 506, and either Mouse IgG2b isotype control (grey histogram), or clone ABM28H9 (purple and blue histograms). Viable cells were used for analysis. {KD}

产品信息

12-9835-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

ABM28H9

抗原

Recombinant fragment of human MAVS (aa 72-296)

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偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2815055

产品详细信息

Description: This ABM28H9 monoclonal antibody recognizes human MAVS, a mitochondrial membrane protein also known as VISA, CARDIF or IPS-1.

Applications Reported: This ABM18H9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This ABM18H9 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of HEL cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at www.thermofisher.com/flowprotocols . This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor 450 is an alternative to Pacific Blue. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

靶标信息

Two distinct signaling pathways activate the host innate immunity against viral infection. One pathway is reliant on members of the Toll-like receptor (TLR) family while the other uses the RNA helicase RIG-I as a receptor for intracellular viral double-stranded RNA as a trigger for the immune response. MAVS is a mitochondrial membrane protein that was identified as a critical component in the IFN beta signaling pathways that recruits IRF-3 to RIG-I, leading to its activation and that of NF-kappa-B. MAVS is also thought to interact with other components of the innate immune pathway such as the TLR adapter protein TRIF, TRAF2 and TRAF6. MAVS also interacts with the IKK-alpha, IKK-beta and IKK-iota kinases through its C-terminal region. Cleavage of this region by the Hepatitis C virus (HCV) protease allows HCV to escape the host immune system. Multiple isoforms of MAVS are known to exist.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CARD adapter inducing interferon beta; CARD adaptor inducing IFN-beta; Cardif; IFN-B promoter stimulator 1; Interferon beta promoter stimulator protein 1; IPS-1; MAVS; Mitochondrial antiviral-signaling protein; Putative NF-kappa-B-activating protein 031N; virus-induced signaling adaptor; Virus-induced-signaling adapter; VISA

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基因别名: CARDIF; IPS-1; IPS1; KIAA1271; MAVS; VISA

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UniProt ID: (Human) Q7Z434

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Entrez Gene ID: (Human) 57506

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功能

signal transducer activity protein binding protein kinase binding CARD domain binding

参与通路