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Agrisera
MC4/Metacaspase-4 Polyclonal Antibody
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MC4/Metacaspase-4 Antibody (AS19-4318) in WB
10 day old Arabidopsis thaliana seedlings, Ler wild type (WT), Col-0 (WT) and MC 4 T-DNA insertion mutants Ler mc 4 and Col-0 mc 4. Grown on 1&(8)260;2 MS medium (no addition of sugar). • Complete seedlings were grinded on liquid nitrogen and tissue powder was immediately mixed with denaturing LaemmLi buffer preheated at 70 °C and further denatured at 70 °C for 10 min (= 0 minutes of wounding). Alternatively, tissue powder was kept at room temperature for 5 minutes and mixed with denaturing LaemmLi buffer preheated at 70 °C and further den... View More
产品信息
AS19-4318
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana MC4.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
Metacaspase-4 (MC4) encodes a cytosolic, Ca2+-responsive cysteine endopeptidase best characterized in Arabidopsis thaliana, where it acts as a damage-activated protease linking loss of plasma membrane integrity to rapid processing of intracellular immune peptides. MC4 is synthesized as an inactive zymogen whose core architecture comprises a caspase-like peptidase C14 domain and an extended internal linker region that enforces autoinhibition by occluding substrate access and positioning a linker lysine residue between catalytic residues; elevated Ca2+ triggers conformational switching and multiple autoprocessing events within the linker to generate an active enzyme. Biochemically, Arabidopsis type II metacaspases including MC4 cleave substrates after basic residues (arginine/lysine), rather than after aspartate as in animal caspases, consistent with a distinct substrate specificity in plants. Functionally, MC4 is necessary and sufficient for maturation of the wound signal Pep1 from its cytosolic precursor PROPEP1 following damage-induced Ca2+ influx, thereby promoting local defense signaling; more broadly, MC4 has been implicated as a stress-responsive protease contributing to regulated cell death and immune activation pathways in planta.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT1G79340; AtMC4; AtMCP2d; MC4; MCP2d; metacaspase 2d; YUP8H12R.4; YUP8H12R_4
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