Invitrogen

MCT1 Monoclonal Antibody (P14612)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2个已发表图片

5 篇参考文献

查看其他20个MCT1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

MCT1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 10

MCT1 Antibody (MA5-18288) in ICC/IF

MCT1 Monoclonal Antibody (P14612) detects MCT1 protein at cell membrane by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCT1 protein stained by MCT1 Monoclonal Antibody (P14612) (Product # MA5-18288) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

MCT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

MCT1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with MCT1 siRNA and loss of signal was observed in Western Blot using MCT1 Monoclonal Antibody (P14612) (Product # MA5-18288). {KD}

产品信息

MA5-18288

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看4篇已发表文章 4篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

P14612

抗原

Recombinant fragment corresponding to a region within amino acids 436 and 500 of MCT1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7, with 20% glycerol

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient

RRID

AB_2539662

产品详细信息

Recommended positive controls: 293T.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

SLC16A1 is a monocarboxylate transporter (MCT1) that mediates the movement of lactate and pyruvate across cell membranes. Import and export of these substrates by tissues such as erythrocytes, muscle, intestine, and kidney are ascribed largely to the action of a proton-coupled MCT.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Pleiotropic effects of Syntaxin16 identified by gene editing in cultured adipocytes.

Frontiers in cell and developmental biology

Bremner SK,Al Shammari WS,Milligan RS,Hudson BD,Sutherland C,Bryant NJ,Gould GW

Published figure using MCT1 monoclonal antibody (Product # MA5-18288) in Western Blot

Tue Dec 06 00:00:00 EST 2022

Using the Cancer Dependency Map to Identify the Mechanism of Action of a Cytotoxic Alkenyl Derivative from the Fruit of Choerospondias axillaris.

Journal of natural products

Kil YS,Risinger AL,Petersen CL,Liang H,Grkovic T,O'Keefe BR,Mooberry SL,Cichewicz RH

MA5-18288 was used in Western Blot to identify a cytotoxic extract from Choerospondias axillaris and elucidate it's functional target using the cancer dependency database.

Fri Mar 27 00:00:00 EDT 2020

Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium.

Scientific data

Lee SH,Hadipour-Lakmehsari S,Kim DH,Di Paola M,Kuzmanov U,Shah S,Lee JJ,Kislinger T,Sharma P,Oudit GY,Gramolini AO

Published figure using MCT1 monoclonal antibody (Product # MA5-18288) in Immunocytochemistry

Tue Dec 01 00:00:00 EST 2020

Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium.

Scientific data

Lee SH,Hadipour-Lakmehsari S,Kim DH,Di Paola M,Kuzmanov U,Shah S,Lee JJ,Kislinger T,Sharma P,Oudit GY,Gramolini AO

Published figure using MCT1 monoclonal antibody (Product # MA5-18288) in Immunocytochemistry

Tue Dec 01 00:00:00 EST 2020

Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium.

Scientific data

Lee SH,Hadipour-Lakmehsari S,Kim DH,Di Paola M,Kuzmanov U,Shah S,Lee JJ,Kislinger T,Sharma P,Oudit GY,Gramolini AO

Published figure using MCT1 monoclonal antibody (Product # MA5-18288) in Immunocytochemistry

Tue Dec 01 00:00:00 EST 2020

Bioinformatic analysis of membrane and associated proteins in murine cardiomyocytes and human myocardium.

Scientific data

Lee SH,Hadipour-Lakmehsari S,Kim DH,Di Paola M,Kuzmanov U,Shah S,Lee JJ,Kislinger T,Sharma P,Oudit GY,Gramolini AO

Published figure using MCT1 monoclonal antibody (Product # MA5-18288) in Immunocytochemistry

Tue Dec 01 00:00:00 EST 2020

Interfering cellular lactate homeostasis overcomes Taxol resistance of breast cancer cells through the microRNA-124-mediated lactate transporter (MCT1) inhibition.

Cancer cell international

Hou L,Zhao Y,Song GQ,Ma YH,Jin XH,Jin SL,Fang YH,Chen YC

MA5-18288 was used in Western Blotting to reveal a possible role of miRNA-214-mediated Taxol resistance, contributing to identify novel therapeutic targets against chemoresistant breast cancers.

Sat Apr 09 00:00:00 EDT 2022

生物信息学

蛋白别名: FLJ36745; MCT 1; MGC44475; monocarboxylate transporter; Monocarboxylate transporter 1; SLC16A1 protein; solute carrier family 16 (monocarboxylate transporter), member 1; solute carrier family 16 (monocarboxylic acid transporters), member 1; Solute carrier family 16 member 1; solute carrier family 16, member 1 (monocarboxylic acid transporter 1)

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基因别名: HHF7; MCT; MCT1; MCT1D; SLC16A1

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UniProt ID: (Human) P53985

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Entrez Gene ID: (Human) 6566

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功能

monocarboxylic acid transmembrane transporter activity lactate transmembrane transporter activity mevalonate transmembrane transporter activity symporter activity protein homodimerization activity organic cyclic compound binding transporter

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