Invitrogen

MCT2 Polyclonal Antibody

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MCT2 Antibody in Immunocytochemistry (ICC/IF)

MCT2 Antibody in Immunocytochemistry (ICC/IF)

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MCT2 Antibody (PA5-112712) in ICC/IF

Immunocytochemistry analysis of MCT2 in Hela cells. The sample was incubated with a MCT2 polyclonal antibody (Product # PA5-112712) at a dilution of 1:200, followed by Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MCT2 Antibody in Immunocytochemistry (ICC/IF)

MCT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MCT2 Antibody in Western Blot (WB)

MCT2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

MCT2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of MCT2 in Hela cells. The sample was incubated with a MCT2 polyclonal antibody (Product # PA5-112712) at a dilution of 1:200, followed by Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MCT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of MCT2 in paraffin-embedded human ovarian cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT, then the primary antibody (1% BSA) was incubated at 4°C overnight. The sample was incubated with a MCT2 polyclonal antibody (Product # PA5-112712) at a dilution of 1:200, followed by a goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

MCT2 Antibody in Western Blot (WB) — Western blot analysis of MCT2 in K-562 whole cell lysate and HepG2 whole cell lysate using a MCT2 polyclonal antibody (Product # PA5-112712) at a dilution of 1:2,000. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000.

MCT2 Antibody in Immunohistochemistry (IHC) — Fig. 3. TS-MAP uncovers a divergent surfaceome landscape in patient gliomas. ( A ) TS-MAP was applied in a mixed cohort ( n = 10) of patient gliomas. Shown are MRI (presurgery and postsurgery within 48 h) and histology for pathological-anatomical diagnostics (PAD) according to clinical routine, here exemplified by patient #8ODG with a low-grade ODG and #1GBM representing a high-grade GBM. Freshly resected tumors were biotinylated ex vivo for downstream analyses, as indicated. ( B ) Confocal microscopy shows biotinylation (green) of intact patient tumor. Higher magnification ( Bottom ) indicates surface labeling, which is further supported by Airyscan imaging of disintegrated SCS ( Bottom Right ), showing specific plasma membrane labeling. Top Right shows example of fresh tumor dissection into pieces of 0.3 to 0.5 cm in diameter prior to biotinylation (ruler scale in centimeters). (Scale bars, 20 mum [ Top Left ], 5 mum [ Bottom Left and Right ].) ( C ) FACS quantification of streptavidin-AF488 association with nonbiotinylated (control) and biotinylated (surface) in a representative GBM patient tissue SCS. ( D ) TS-MAP was applied in a pilot cohort of 10 freshly resected patient tumors, including low-grade ODG (WHO grade II), high-grade anaplastic astrocytoma (AA, WHO grade III), primary glioblastoma (GBM, WHO grade IV), recurrent GBM (GBMr), and gliosarcoma (WHO grade IV). Heatmap of SURFME protein abundance demonstrates divergent expression profile among patient tumors. ( E

产品信息

PA5-112712

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:5,000

-

免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

1:20-1:200

-

免疫细胞化学 (ICC/IF)

1:50-1:200

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酶联免疫吸附实验 (ELISA)

Assay-dependent

-

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human Monocarboxylate transporter 2 protein (197-279AA)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% ProClin 300

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

RRID

AB_2867447

靶标信息

Tissues with few or no mitochondria, such as erythrocytes and tumor cells, depend largely on glycolysis to generate ATP. The major end products of glycolysis, pyruvate and lactate, must be eliminated from these cells to enable continued glycolytic flux and prevent toxic effects. H+/monocarboxylate transporters mediate the transport of lactate and pyruvate. Human MCT2 has a high affinity for the transport of pyruvate.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Landscape of surfaceome and endocytome in human glioma is divergent and depends on cellular spatial organization.

Proceedings of the National Academy of Sciences of the United States of America

Governa V,Talbot H,Gonçalves de Oliveira K,Cerezo-Magaña M,Bång-Rudenstam A,Johansson MC,Månsson AS,Forsberg-Nilsson K,Marko-Varga G,Enríquez Pérez J,Darabi A,Malmström J,Bengzon J,Welinder C,Belting M

Published figure using MCT2 polyclonal antibody (Product # PA5-112712) in Immunohistochemistry

Tue Mar 01 00:00:00 EST 2022

Landscape of surfaceome and endocytome in human glioma is divergent and depends on cellular spatial organization.

Proceedings of the National Academy of Sciences of the United States of America

Governa V,Talbot H,Gonçalves de Oliveira K,Cerezo-Magaña M,Bång-Rudenstam A,Johansson MC,Månsson AS,Forsberg-Nilsson K,Marko-Varga G,Enríquez Pérez J,Darabi A,Malmström J,Bengzon J,Welinder C,Belting M

Published figure using MCT2 polyclonal antibody (Product # PA5-112712) in Immunohistochemistry

Tue Mar 01 00:00:00 EST 2022

Landscape of surfaceome and endocytome in human glioma is divergent and depends on cellular spatial organization.

Proceedings of the National Academy of Sciences of the United States of America

Governa V,Talbot H,Gonçalves de Oliveira K,Cerezo-Magaña M,Bång-Rudenstam A,Johansson MC,Månsson AS,Forsberg-Nilsson K,Marko-Varga G,Enríquez Pérez J,Darabi A,Malmström J,Bengzon J,Welinder C,Belting M

Published figure using MCT2 polyclonal antibody (Product # PA5-112712) in Immunohistochemistry

Tue Mar 01 00:00:00 EST 2022

Landscape of surfaceome and endocytome in human glioma is divergent and depends on cellular spatial organization.

Proceedings of the National Academy of Sciences of the United States of America

Governa V,Talbot H,Gonçalves de Oliveira K,Cerezo-Magaña M,Bång-Rudenstam A,Johansson MC,Månsson AS,Forsberg-Nilsson K,Marko-Varga G,Enríquez Pérez J,Darabi A,Malmström J,Bengzon J,Welinder C,Belting M

Published figure using MCT2 polyclonal antibody (Product # PA5-112712) in Immunohistochemistry

Tue Mar 01 00:00:00 EST 2022

Landscape of surfaceome and endocytome in human glioma is divergent and depends on cellular spatial organization.

Proceedings of the National Academy of Sciences of the United States of America

Governa V,Talbot H,Gonçalves de Oliveira K,Cerezo-Magaña M,Bång-Rudenstam A,Johansson MC,Månsson AS,Forsberg-Nilsson K,Marko-Varga G,Enríquez Pérez J,Darabi A,Malmström J,Bengzon J,Welinder C,Belting M

Published figure using MCT2 polyclonal antibody (Product # PA5-112712) in Immunohistochemistry

Tue Mar 01 00:00:00 EST 2022

生物信息学

蛋白别名: MCT 2; monocarboxylate transporter; Monocarboxylate transporter 2; solute carrier family 16 (monocarboxylate transporter), member 7; Solute carrier family 16 member 7; solute carrier family 16, member 7 (monocarboxylic acid transporter 2)

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基因别名: MCT2; SLC16A7

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UniProt ID: (Human) O60669

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Entrez Gene ID: (Human) 9194

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pyruvate secondary active transmembrane transporter activity monocarboxylic acid transmembrane transporter activity lactate transmembrane transporter activity symporter activity pyruvate transmembrane transporter activity transporter

参与通路

lactate transmembrane transport plasma membrane lactate transport pyruvate transmembrane transport