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Invitrogen
MCU Recombinant Rabbit Monoclonal Antibody (PSH13-74)
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图: 1 / 11
MCU Antibody (MA562924) in ICC/IF
Immunofluorescence analysis of MCU using NIH/3T3. The cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. The cells were co-labeled with MCU Recombinant Rabbit Monoclonal Antibody (PSH13-74) (Product # MA562924) (green) and beta tubulin (red) at 1:100 dilution in 1% BSA in PBST overnight at 4 degrees Celsius and then with iFluor™ 488 Goat Anti-Rabbit IgG H... View More
产品信息
MA562924
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PSH13-74
抗原
Recombinant protein within human MCU aa 51-233.
偶联物
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Positive Control: HCT 116 cell lysate, PANC-1 cell lysate, A549 cell lysate, HeLa cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, PC-12 cell lysate, Mouse colon tissue lysate, Mouse heart tissue lysate, Mouse testis tissue lysate, Rat colon tissue lysate, Rat heart tissue lysate, human colon tissue, human colon cancer tissue, HCT 116, NIH/3T3, C6.
Tissue Specificity: Tissue enhanced (tongue)
Subcellular Location: Mitochondrion inner membrane.
Sequence Similarities: 95% Mouse/Rat
Predicted band size: 40 kDa
靶标信息
Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex). Activity is regulated by MICU1 and MICU2. At low Ca(2+) levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca(2+) levels MICU1 increases MCU activity. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells. participates in mitochondrial permeability transition during ischemia-reperfusion injury. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults. Regulates synaptic vesicle endocytosis kinetics in central nerve terminal. Involved in antigen processing and presentation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Calcium uniporter protein, mitochondrial; coiled-coil domain containing 109A; Coiled-coil domain-containing protein 109A; HsMCU; unnamed protein product
基因别名: 2010012O16Rik; AV064928; C10orf42; CCDC109A; D130073L02Rik; Gm64; HsMCU; MCU
UniProt ID: (Human) Q8NE86, (Mouse) Q3UMR5
Entrez Gene ID: (Human) 90550, (Rat) 294560, (Mouse) 215999
mitochondrial calcium ion transport
calcium-mediated signaling
positive regulation of insulin secretion
protein complex oligomerization
calcium ion transmembrane import into mitochondrion
glucose homeostasis
mitochondrial calcium ion homeostasis
positive regulation of mitochondrial calcium ion concentration
generation of precursor metabolites and energy
ion transport
calcium ion transport
cell proliferation
cell migration
ion transmembrane transport
protein oligomerization
calcium ion transmembrane transport
cellular response to calcium ion starvation
positive regulation of neutrophil chemotaxis
positive regulation of mitochondrial fission
transport
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