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Invitrogen
MDH2 Monoclonal Antibody (5D8C1)
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MDH2 Antibody (MA5-49280) in ICC/IF
Immunocytochemistry analysis of MDH2 in Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in MDH2 Monoclonal antibody (Product # MA5-49280) using a dilution of 5 μg/mL. Cy3 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label... View More
产品信息
MA5-49280
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
5D8C1
抗原
E.coli-derived human MDH2 recombinant protein (Position: A9-L223).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human U-87MG whole cell, human A431 whole cell. IHC: human breast cancer tissue, human cervical cancer tissue, human squamous cell carcinoma tissue. ICC/IF: Hela cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: malate dehydrogenase 2, NAD (mitochondrial); Malate dehydrogenase, mitochondrial; testicular tissue protein Li 120
基因别名: M-MDH; MDH; MDH2; MGC:3559; MOR1
UniProt ID: (Human) P40926
Entrez Gene ID: (Human) 4191
