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Invitrogen
ME1 Monoclonal Antibody (5E5F7)
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ME1 Antibody (MA5-49254) in IHC (P)
Immunohistochemistry analysis of ME1 in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with ME1 Monoclonal antibody (Product # MA5-49254) using a dilution of 2 μg/mL overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG w... View More
产品信息
MA5-49254
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
5E5F7
抗原
E.coli-derived human ME1 recombinant protein (Position: M1-Q572).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human A549 whole cell, human Hela whole cell, rat testis tissue, rat brain tissue, mouse testis tissue, mouse brain tissue. IHC: human liver cancer tissue, human spleen tissue, rat testis tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
ME1 encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cytosolic malic enzyme 1; malate dehydrogenase; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)); malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+); Malic enzyme 1; malic enzyme 1, NADP(+)-dependent, cytosolic; malic enzyme 1, soluble; Malic enzyme, cytoplasmic; malic enzyme, supernatant; NADP-dependent malic enzyme; NADP-ME; pyruvic-malic carboxylase
基因别名: D9Ertd267e; HUMNDME; Mdh-1; ME1; MES; Mod-1; MOD1
UniProt ID: (Human) P48163, (Mouse) P06801
Entrez Gene ID: (Human) 4199, (Mouse) 17436, (Rat) 24552
malic enzyme activity
malate dehydrogenase (decarboxylating) (NAD+) activity
malate dehydrogenase (decarboxylating) (NADP+) activity
oxaloacetate decarboxylase activity
electron carrier activity
manganese ion binding
ADP binding
NADP binding
NAD binding
protein binding
oxidoreductase activity
metal ion binding
oxidoreductase
