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Invitrogen
MED12 Polyclonal Antibody
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MED12 Antibody (PA5-51852) in ICC/IF
Immunofluorescence analysis of MED12 was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MED12 Polyclonal Antibody (Product # PA5-51852) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32... View More
产品信息
PA5-51852
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
偶联物
形式
Liquid
浓度
0.1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 40% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: LPTTMTGVMG LEPSSYKTSV YRQQQPAVPQ GQRLRQQLQA KIQSQGMLGQ SSVHQMTPSS SYGLQTSQGY TPYVSHVGLQ QHTGPAGTMV PPSYSSQPYQ STHPSTNPTL VDPTRHLQQR PSGYVHQQAP TYGHGLTS
Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 97%.
靶标信息
The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; human opposite paired; Mediator complex subunit 12; Mediator of RNA polymerase II transcription subunit 12; mediator of RNA polymerase II transcription, subunit 12 homolog; OPA-containing protein; putative mediator subunit 12; Thyroid hormone receptor-associated protein complex 230 kDa component; thyroid hormone receptor-associated protein, 230 kDa subunit; Trap230; trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit); Trinucleotide repeat-containing gene 11 protein
基因别名: ARC240; CAGH45; FGS1; HOPA; KIAA0192; MED12; MED12S; OHDOX; OKS; OPA1; TNRC11; TRAP230
UniProt ID: (Human) Q93074
Entrez Gene ID: (Human) 9968
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II transcription cofactor activity
RNA polymerase II transcription coactivator activity
chromatin binding
transcription cofactor activity
transcription coactivator activity
receptor activity
protein binding
beta-catenin binding
protein C-terminus binding
protein domain specific binding
ligand-dependent nuclear receptor transcription coactivator activity
vitamin D receptor binding
thyroid hormone receptor binding
ubiquitin protein ligase activity
general transcription factor
neural tube closure
transcription initiation from RNA polymerase II promoter
endoderm development
heart development
oligodendrocyte development
Schwann cell development
protein ubiquitination
stem cell population maintenance
spinal cord development
negative regulation of Wnt signaling pathway
intracellular steroid hormone receptor signaling pathway
androgen receptor signaling pathway
post-anal tail morphogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic neurocranium morphogenesis
canonical Wnt signaling pathway
Wnt signaling pathway, planar cell polarity pathway
axis elongation involved in somitogenesis
embryonic brain development
