Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
MED12 Recombinant Rabbit Monoclonal Antibody (PSH06-67)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
图: 1 / 4
MED12 Antibody (MA5-56388) in WB
Western blot was performed using MED12 Recombinant Rabbit Monoclonal Antibody (PSH06-67) (Product # MA5-56388) and a 250 kDa band corresponding to MED12 was observed across cell lines and tissue tested. Whole cells and tissue extracts (20 µg lysate) of A549 (Lane 1), HeLa (Lane 2), K-562 (Lane 3), LNCaP (Lane 4), Jurkat (Lane 5), HCT 116 (Lane 6), Caco-2 (Lane 7), NIH/3T3 (Lane 8), C2C12 (Lane 9), PC-12 (Lane 10) and rat placenta (Lane 11) were electrophoresed using 4-20% SDS-PAGE gel. Resolved proteins were transferred onto a PVDF membran... View More
产品信息
MA5-56388
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PSH06-67
抗原
Recombinant protein within human MED12 aa 1,001-1,350.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 40% glycerol, 0.1% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
Positive Control: A549 cell lysate, HeLa cell lysate, K-562 cell lysate, LNCaP cell lysate, Jurkat cell lysate, HCT 116 cell lysate, Caco-2 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, Rat placenta tissue lysate.
Tissue Specificity: Low tissue specificity.
Subcellular Location: Nucleus.
Sequence Similarities: 99% Mouse/Rat.
Predicted band size: 243 kDa.
靶标信息
The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; human opposite paired; Mediator complex subunit 12; Mediator of RNA polymerase II transcription subunit 12; mediator of RNA polymerase II transcription, subunit 12 homolog; OPA-containing protein; OPA-containing protein 1; putative mediator subunit 12; Thyroid hormone receptor-associated protein complex 230 kDa component; thyroid hormone receptor-associated protein, 230 kDa subunit; Trap230; trinucleotide repeat containing 11 (THR-associated protein); trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit); Trinucleotide repeat-containing gene 11 protein
基因别名: 230kDa; ARC240; CAGH45; FGS1; HOPA; KIAA0192; MED12; MED12S; Mopa; OHDOX; OKS; OPA-1; OPA1; TNRC11; TRAP230
UniProt ID: (Human) Q93074, (Mouse) A2AGH6
Entrez Gene ID: (Human) 9968, (Rat) 679693, (Mouse) 59024
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II transcription cofactor activity
RNA polymerase II transcription coactivator activity
chromatin binding
transcription cofactor activity
transcription coactivator activity
receptor activity
protein binding
beta-catenin binding
protein C-terminus binding
protein domain specific binding
ligand-dependent nuclear receptor transcription coactivator activity
vitamin D receptor binding
thyroid hormone receptor binding
ubiquitin protein ligase activity
transcriptional activator activity, RNA polymerase II transcription factor binding
transcription factor binding
general transcription factor
neural tube closure
transcription initiation from RNA polymerase II promoter
endoderm development
heart development
oligodendrocyte development
Schwann cell development
protein ubiquitination
stem cell population maintenance
spinal cord development
negative regulation of Wnt signaling pathway
intracellular steroid hormone receptor signaling pathway
androgen receptor signaling pathway
post-anal tail morphogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic neurocranium morphogenesis
canonical Wnt signaling pathway
Wnt signaling pathway, planar cell polarity pathway
axis elongation involved in somitogenesis
embryonic brain development
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription, DNA-templated
