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Invitrogen
MEKK3 Recombinant Rabbit Monoclonal Antibody (9N3Y9)
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MEKK3 Antibody (MA5-38090) in WB
Western blot analysis of MEKK3 in extracts of various cell lines. Samples were incubated with MEKK3 Monoclonal antibody (Product # MA5-38090) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
产品信息
MA5-38090
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
9N3Y9
抗原
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEKK3 (Q99759)
偶联物
形式
Liquid
浓度
1.05 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive Samples: HeLa, NIH/3T3, Jurkat, SH-SY5Y, Mouse spleen
Immunogen sequence: MDEQEALNSI MNDLVALQMN RRHRMPGYET MKNKDTGHSN RQSDVRIKFE HNGERRIIAF SRPVKYEDVE HKVTTVFGQP LDLHYMNNEL SILLKNQDDL
靶标信息
MAP3K3 (MEKK3) directly regulates MAPK8 and extracellular signal-regulated protein kinase (ERK) pathways by phosphorylating multiple MAP2Ks. Northern blot analysis revealed a 4. 6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: MAP/ERK kinase kinase 3; MAPK/ERK kinase kinase 3; MEK kinase 3; MEKK 3; mitogen activated protein kinase kinase kinase 3; Mitogen-activated protein kinase kinase kinase 3
基因别名: AW548911; MAP3K3; MAPKKK3; MEKK3; mKIAA4031
UniProt ID: (Human) Q99759, (Mouse) Q61084
Entrez Gene ID: (Human) 4215, (Mouse) 26406
