Invitrogen

METTL14 Polyclonal Antibody

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3 篇参考文献

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问题与答案

问题与答案

METTL14 Antibody in Immunocytochemistry (ICC/IF)

METTL14 Antibody in Immunocytochemistry (ICC/IF)

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METTL14 Antibody (PA5-58204) in ICC/IF

Immunofluorecent analysis of METTL14 in human cell line A-431 using METTL14 Polyclonal Antibody (Product # PA5-58204). Staining shows localization to nucleoplasm.

METTL14 Antibody in Immunocytochemistry (ICC/IF)

METTL14 Antibody in Immunohistochemistry (IHC)

METTL14 Antibody in Immunohistochemistry (IHC)

METTL14 Antibody in Immunohistochemistry (IHC)

METTL14 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

METTL14 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

METTL14 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

METTL14 Antibody in Immunocytochemistry (ICC/IF)

METTL14 Antibody in Immunocytochemistry (ICC/IF)

METTL14 Antibody in Western Blot (WB)

METTL14 Antibody in Western Blot (WB)

METTL14 Antibody in Western Blot (WB)

METTL14 Antibody in Western Blot (WB)

METTL14 Antibody in Western Blot, Immunoprecipitation (WB, IP)

METTL14 Antibody

Product Image Gallery

METTL14 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorecent analysis of METTL14 in human cell line A-431 using METTL14 Polyclonal Antibody (Product # PA5-58204). Staining shows localization to nucleoplasm.

METTL14 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of METTL14 in human kidney using a METTL14 Polyclonal Antibody (Product # PA5-58204) shows moderate to strong nuclear positivity in glomeruli and cells in tubules.

METTL14 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of METTL14 in human testis using a METTL14 Polyclonal Antibody (Product # PA5-58204) shows moderate to strong nuclear positivity in cells in seminiferous ducts.

METTL14 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of METTL14 in human fallopian tube using a METTL14 Polyclonal Antibody (Product # PA5-58204) shows strong nuclear positivity in glandular cells.

METTL14 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — FIGURE 5 METTL14 overexpression ameliorates senescence. (a) Western blot analysis of METTL3/14 and p21 levels in lenti-METTL14 (lenti-M14)-infected HSFs at P23. (b) Representative images of SA-beta-Gal staining of lenti-M14-infected HSFs at the indicated passages. Scale bar, 100 um. (c) Quantification of the beta-Gal-positive cells in (b) based on 10 randomly chosen views for each group. The data represent the means +- SEM . * p < 0.05. (d) Representative immunofluorescence images of METTL14 and Lamin A expression in lenti-METTL14-infected HG155 at P23. Scale bar, 100 um. (e) The percentage of cells with a normal nuclear shape (%) based on staining from 10 randomly chosen views for each group in (d). The data represent the means +- SEM . ** p < 0.01. (f) Representative immunofluorescence images of METTL14 and H3K9me3 staining in lenti-METTL14-infected HG155 at P23. Scale bar, 100 um. (g) Quantification of (f). The percentage of H3K9me3-positive cells (%) based on 10 randomly chosen views for each group. The data represent the means +- SEM . ** p < 0.01. (h) Western blot analysis of METTL3/14, p21, and p16 levels in adeno-associated virus (AAV)-METTL14-infected Zmpste24 -/- and Zmpste24 +/+ MEFs at P6. (i) SA-beta-Gal staining of AAV-METTL14-infected Zmpste24 -/- MEFs at P6. Scale bar, 100 um. (j) Quantification of the SA-beta-Gal-positive cells in (i) from 10 randomly chosen views for each group. The data represent the means +- SEM . * p < 0.05. The data represent three indep

METTL14 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — FIGURE 3 Lamin A abnormality destabilizes METTL14 protein. (a, b) Representative immunofluorescence images showing METTL14/3 and Lamin A expression in skin fibroblasts derived from individual HGPS patients (HG122 and HG155) and normal human skin fibroblasts (F2S). Scale bar, 50 um. (c) Western blot analysis of METTL3/14 protein levels in Zmpste24 +/+ , Zmpste24 -/- , Lmna +/+ , and Lmna -/- MEFs. The data represent three independently derived sets of MEFs in separate experiments. (d, e) Western blot analysis of METTL3/14 protein levels in HG122, HG155, and F2S cells at different passages. (f, g) Western blot analysis of METTL3/14 expression in Lmna -/- (f) and Zmpste24 -/- (g) MEFs compared with WT cells. Cells were treated with cycloheximide (CHX) and/or MG132 (MG) for indicated time. Quantification was performed by Image J (r) . The data represent the means +- SEM . * p < 0.05

METTL14 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — 3 FIGURE Lamin A abnormality destabilizes METTL14 protein. (a, b) Representative immunofluorescence images showing METTL14/3 and Lamin A expression in skin fibroblasts derived from individual HGPS patients (HG122 and HG155) and normal human skin fibroblasts (F2S). Scale bar, 50 um. (c) Western blot analysis of METTL3/14 protein levels in Zmpste24 +/+ , Zmpste24 -/- , Lmna +/+ , and Lmna -/- MEFs. The data represent three independently derived sets of MEFs in separate experiments. (d, e) Western blot analysis of METTL3/14 protein levels in HG122, HG155, and F2S cells at different passages. (f, g) Western blot analysis of METTL3/14 expression in Lmna -/- (f) and Zmpste24 -/- (g) MEFs compared with WT cells. Cells were treated with cycloheximide (CHX) and/or MG132 (MG) for indicated time. Quantification was performed by Image J (r) . The data represent the means +- SEM . * p < 0.05

METTL14 Antibody in Immunocytochemistry (ICC/IF) — 2 FIGURE The nuclear localization of METTL3/14 requires Lamin A. (a) Representative immunofluorescence analysis of METTL3, METTL14, and SC35 foci in Lmna +/+ and Lmna -/- MEFs. (b) Representative immunofluorescence analysis of GFP-METTL14 (GFP-M14) in Lmna +/+ and Lmna -/- MEFs with or without DsRed-Lamin A (Red-LA). (c) Representative immunofluorescence analysis of METTL14 and SC35 foci in Zmpste24 +/+ and Zmpste24 -/- MEFs. (d) The quantitative analysis of (c). *** p < 0.001. (e) Co-IP and Western blot analysis of the interaction between SC35 and METTL3/14 in the presence of Lamin A, Progerin, and prelamin A. Scale bars, 10 um

METTL14 Antibody in Immunocytochemistry (ICC/IF) — FIGURE 2 The nuclear localization of METTL3/14 requires Lamin A. (a) Representative immunofluorescence analysis of METTL3, METTL14, and SC35 foci in Lmna +/+ and Lmna -/- MEFs. (b) Representative immunofluorescence analysis of GFP-METTL14 (GFP-M14) in Lmna +/+ and Lmna -/- MEFs with or without DsRed-Lamin A (Red-LA). (c) Representative immunofluorescence analysis of METTL14 and SC35 foci in Zmpste24 +/+ and Zmpste24 -/- MEFs. (d) The quantitative analysis of (c). *** p < 0.001. (e) Co-IP and Western blot analysis of the interaction between SC35 and METTL3/14 in the presence of Lamin A, Progerin, and prelamin A. Scale bars, 10 um

METTL14 Antibody in Western Blot (WB) — 5 FIGURE METTL14 overexpression ameliorates senescence. (a) Western blot analysis of METTL3/14 and p21 levels in lenti-METTL14 (lenti-M14)-infected HSFs at P23. (b) Representative images of SA-beta-Gal staining of lenti-M14-infected HSFs at the indicated passages. Scale bar, 100 um. (c) Quantification of the beta-Gal-positive cells in (b) based on 10 randomly chosen views for each group. The data represent the means +- SEM . * p < 0.05. (d) Representative immunofluorescence images of METTL14 and Lamin A expression in lenti-METTL14-infected HG155 at P23. Scale bar, 100 um. (e) The percentage of cells with a normal nuclear shape (%) based on staining from 10 randomly chosen views for each group in (d). The data represent the means +- SEM . ** p < 0.01. (f) Representative immunofluorescence images of METTL14 and H3K9me3 staining in lenti-METTL14-infected HG155 at P23. Scale bar, 100 um. (g) Quantification of (f). The percentage of H3K9me3-positive cells (%) based on 10 randomly chosen views for each group. The data represent the means +- SEM . ** p < 0.01. (h) Western blot analysis of METTL3/14, p21, and p16 levels in adeno-associated virus (AAV)-METTL14-infected Zmpste24 -/- and Zmpste24 +/+ MEFs at P6. (i) SA-beta-Gal staining of AAV-METTL14-infected Zmpste24 -/- MEFs at P6. Scale bar, 100 um. (j) Quantification of the SA-beta-Gal-positive cells in (i) from 10 randomly chosen views for each group. The data represent the means +- SEM . * p < 0.05. The data represent three indep

METTL14 Antibody in Western Blot (WB) — FIGURE 4 METTL3/14 decline accelerates senescence. (a, b) Western blot analysis of the METTL3/14 and p21 WIF1 levels with subsequent passaging of human skin fibroblasts (HSFs) (a) and wild-type (WT) mouse embryonic fibroblasts (MEFs) (b). (c) Western blot analysis of METTL3/14, p21, and p16 protein levels in HSFs (P17) treated with the indicated siRNAs. (d) Representative images of SA-beta-Gal staining in HSFs (P17) treated with the indicated siRNAs. The percentage of SA-beta-Gal-positive cells is shown. The data represent the means +- SEM . Scale bar, 100 um. (e) Western blot analysis of METTL14, p21, and p16 levels in MEFs (P3) treated with the indicated siRNAs. (f) Representative images of SA-beta-Gal staining in WT MEFs treated with the indicated siRNAs. The percentage of SA-beta-Gal-positive cells is shown. The data represent the means +- SEM . Scale bar, 100 um

METTL14 Antibody in Western Blot (WB) — 4 FIGURE METTL3/14 decline accelerates senescence. (a, b) Western blot analysis of the METTL3/14 and p21 WIF1 levels with subsequent passaging of human skin fibroblasts (HSFs) (a) and wild-type (WT) mouse embryonic fibroblasts (MEFs) (b). (c) Western blot analysis of METTL3/14, p21, and p16 protein levels in HSFs (P17) treated with the indicated siRNAs. (d) Representative images of SA-beta-Gal staining in HSFs (P17) treated with the indicated siRNAs. The percentage of SA-beta-Gal-positive cells is shown. The data represent the means +- SEM . Scale bar, 100 um. (e) Western blot analysis of METTL14, p21, and p16 levels in MEFs (P3) treated with the indicated siRNAs. (f) Representative images of SA-beta-Gal staining in WT MEFs treated with the indicated siRNAs. The percentage of SA-beta-Gal-positive cells is shown. The data represent the means +- SEM . Scale bar, 100 um

METTL14 Antibody in Western Blot (WB) — FIGURE 1 Lamin A interacts with METTL3/14. (a-b) Co-immunoprecipitation (Co-IP) and Western blot analysis of the interactions between FLAG-Lamin A and HA-METTL14 (a); and FLAG-Lamin A HA-METTL3 (b) in HEK293 cells. (c-d) Co-IP and Western blot analysis of the endogenous interaction between Lamin A and METTL14 (c) and Lamin A and METTL3 (d) in MEFs

METTL14 Antibody in Western Blot, Immunoprecipitation (WB, IP) — 1 FIGURE Lamin A interacts with METTL3/14. (a-b) Co-immunoprecipitation (Co-IP) and Western blot analysis of the interactions between FLAG-Lamin A and HA-METTL14 (a); and FLAG-Lamin A HA-METTL3 (b) in HEK293 cells. (c-d) Co-IP and Western blot analysis of the endogenous interaction between Lamin A and METTL14 (c) and Lamin A and METTL3 (d) in MEFs

METTL14 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. A-431 cells were transfected with METTL14 siRNA and decrease in signal intensity was observed in western blot application using METTL14 Polyclonal Antibody (PA5-58204). {KD}

Immunogen image

METTL14 Polyclonal Antibody

Immunogen Image METTL14 Polyclonal Antibody

产品信息

PA5-58204

应用

建议稀释比

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免疫印迹 (WB)

-

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免疫组化（石蜡） (IHC (P))

1:1,000-1:2,500

-

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

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免疫沉淀 (IP)

-

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产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human METTL14. Recombinant protein control fragment (Product #RP-96679).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.30 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Immunogen sequence: RSWNMDSRLQ EIRERQKLRR QLLAQQLGAE SADSIGAVLN SKDEQREIAE TRETCRASYD TSAPNAKRKY LDEGETDEDK MEEYKDELEM QQDEE

Highest antigen sequence identity to the following orthologs: Mouse - 92%, Rat - 92%.

靶标信息

KIAA1627 is a probable methyltransferase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: hMETTL14; Methyltransferase-like protein 14; N(6)-adenosine-methyltransferase non-catalytic subunit METTL14

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基因别名: KIAA1627; METTL14

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UniProt ID: (Human) Q9HCE5

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Entrez Gene ID: (Human) 57721

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mRNA binding protein binding mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity S-adenosyl-L-methionine binding RNA processing factor

mRNA splicing, via spliceosome gene expression stem cell population maintenance mRNA destabilization mRNA methylation