Invitrogen

METTL23 Polyclonal Antibody

查看其他3个METTL23抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (1)

METTL23 Antibody in Western Blot (WB)

METTL23 Antibody in Western Blot (WB)

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METTL23 Antibody (PA5-144081) in WB

Western blot analysis of METTL23 using METTL23 antibody (Product # PA5-144081). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human HCT whole cell lysates; Lane 2: human Caco-2 whole cell lysates; Lane 3: human SHY whole cell lysates; Lane 4: human U87 whole cell lysates; Lane 5: mouse GL261 whole cell lysates. After Electrophoresis, proteins were transferred to a PVDF membrane a... View More

METTL23 Antibody in Western Blot (WB)

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METTL23 Antibody in Western Blot (WB) — Western blot analysis of METTL23 using METTL23 antibody (Product # PA5-144081). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human HCT whole cell lysates; Lane 2: human Caco-2 whole cell lysates; Lane 3: human SHY whole cell lysates; Lane 4: human U87 whole cell lysates; Lane 5: mouse GL261 whole cell lysates. After Electrophoresis, proteins were transferred to a PVDF membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL23 antigen affinity purified polyclonal antibody (Product # PA5-144081) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and detected by Licor IR800 rabbit antibody at a dilution of 1:5,000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.

Immunogen image

METTL23 Polyclonal Antibody

Immunogen Image METTL23 Polyclonal Antibody

产品信息

PA5-144081

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5 µg/mL

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Human METTL23 between 11-190

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Positive Control - WB: human HCT whole cell, human Caco-2 whole cell, human SHY whole cell, human U87 whole cell, mouse GL261 whole cell.

靶标信息

The protein encoded by this gene functions as a transcription factor regulator in the transcriptional pathway for human cognition. It is a partner of the alpha subunit of the GA-binding protein transcription factor. Mutations in this gene cause mild autosomal recessive intellectual disability. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Histone-arginine methyltransferase METTL23; Methyltransferase-like protein 23

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基因别名: 1110005A03Rik; 1500035B17Rik; 4933424L15Rik; C17orf95; METTL23; MRT44

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UniProt ID: (Human) Q86XA0, (Mouse) A2AA28

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Entrez Gene ID: (Human) 124512, (Mouse) 74319

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methyltransferase activity rRNA (adenine-N6,N6-)-dimethyltransferase activity molecular_function protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity

biological_process methylation