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METTL3 Antibody (PA5-28178) in ICC/IF
Immunofluorescence analysis of N6-adenosine-methyltransferase catalytic subunit was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with METTL3 Polyclonal Antibody (Product # PA5-28178) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Anti... View More
产品信息
PA5-28178
产品规格
种属反应
Human
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 172 and 453 of Human METTL3
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2545654
产品详细信息
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (80%), Xenopus laevis (81%), Chimpanzee (100%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
METTL3 is the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase; hMETTL3; Methyltransferase-like protein 3; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; mRNA m(6)A methyltransferase; MT-A70; N6-adenosine-methyltransferase 70 kDa subunit; N6-adenosine-methyltransferase catalytic subunit
基因别名: IME4; M6A; METTL3; MT-A70; MTA70; Spo8
UniProt ID: (Human) Q86U44
Entrez Gene ID: (Human) 56339
mRNA splicing, via spliceosome
RNA methylation
adenosine to inosine editing
mRNA processing
circadian rhythm
gene expression
stem cell population maintenance
primary miRNA processing
mRNA destabilization
mRNA methylation
positive regulation of cap-independent translational initiation
primary miRNA methylation
