Invitrogen

MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), Functional Grade, eBioscience™

Name: MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), Functional Grade, eBioscience\u2122
Brand: Invitrogen
SKU: 16-5957-82

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

已发表图片 (10)

MHC Class I (H-2Kd) Antibody in Flow Cytometry (Flow)

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MHC Class I (H-2Kd) Antibody (16-5957-82) in Flow

Communications biology 2021 - Fig. 7 NME1 promotes lung metastasis from patient tumor cells. a Schematic of experimental workflow to investigate the effect of NME1 overexpression on spontaneous metastasis from cultured PDX tumor cells. b The box plot shows normalized gene expression of NME1 in metastatic (red, n = 435 cells) and primary tumor cells (blue, n = 684 cells). The lower and upper hinges correspond to the first and third quart... View More

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产品信息

16-5957-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

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功能检测 (Functional)

Assay-Dependent

抑制试剂 (IA)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

SF1-1.1.1

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10670098

产品详细信息

Description: This SF1-1.1.1 monoclonal antibody reacts with the H-2Kd MHC class I alloantigen. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. b, j, k, p, q, s, and v) has not been observed.

This monoclonal antibody has been reported to block CD8 involvement in TCR-ligand binding.

Applications Reported: This SF1-1.1.1 antibody has been reported for use in functional assays.

Applications Tested: This SF1-1.1.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K(B); H-2K(K); H-2K(Q); MGC184092; MGC7052; MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen

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基因别名: H-2K; H-2K(d); H2-K; H2-K1; K-f

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UniProt ID: (Mouse) P01901

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Entrez Gene ID: (Mouse) 14972

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功能