Invitrogen

CD19 Monoclonal Antibody (SJ25-C1), PE-Alexa Fluor™ 610

Name: CD19 Monoclonal Antibody (SJ25-C1), PE-Alexa Fluor\u2122 610
Brand: Invitrogen
SKU: MHCD1922

高级验证

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5 篇参考文献

查看其他273个CD19抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

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CD19 Antibody (MHCD1922) in Flow

Anti-human CD19 monoclonal antibody (clone SJ25-C1) was used to delineate B-cells in a human peripheral blood lymphocyte gate. PE-Alexa Fluor® 610 is shown, and corresponding negative control profiles represent unstained cells.

Product Image Gallery

CD19 Antibody — Antibody clone (SJ25C1) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in CD19 KO cells (blue histogram) compared to the control Cas9 cells (pink histogram) using CD19 antibody (SJ25C1). Yellow histogram represents staining with the isotype control. {KO}

Immunogen image

Immunogen Image CD19 Monoclonal Antibody (SJ25-C1), PE-Alexa Fluor™ 610

产品信息

MHCD1922

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

SJ25-C1

抗原

Nalm 1 Human cell line

查看免疫原

偶联物

PE-Alexa Fluor™ 610 PE-Alexa Fluor™ 610 PE-Alexa Fluor™ 610

激发/发射光谱

567/627 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with 4mg/mL BSA

内含物

0.1% sodium azide

保存条件

4°C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_10373379

产品详细信息

The R-phycoerythrin (PE)-Cy7 tandem conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a 488 nm laser without occupying traditional channels associated with FITC and PE.

靶标信息

CD19 is a member of the immunoglobulin superfamily, characterized by two Ig-like domains, and is expressed on B cells throughout all stages of development, excluding terminally differentiated plasma cells. It is also expressed on follicular dendritic cells and has been observed on myeloid leukemia cells, particularly those of monocytic lineage. CD19 is considered the earliest and broadest B cell-restricted antigen, and its expression is found in all B cell precursor leukemias. CD19 forms a multimolecular complex with CD21, CD81, Leu13, MHC class II, and the B cell receptor (BCR), playing a crucial role in B cell signaling. As a signal-amplifying coreceptor for the BCR, CD19 lowers the threshold for antigen receptor-dependent stimulation, allowing B cells to respond specifically and sensitively to various antigens through low-affinity antigen receptors. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. Beyond its role as a BCR coreceptor, CD19 can also signal independently of BCR co-ligation, serving as a central regulatory component upon which multiple signaling pathways converge. This makes CD19 an important functional regulator of both normal and malignant B cell proliferation. Mutations in the CD19 gene can result in hypogammaglobulinemia, a condition characterized by low levels of immunoglobulins, while CD19 overexpression can lead to B cell hyperactivity. CD19 is expressed on 100% of peripheral B cells, as defined by the expression of kappa or lambda light chains, underscoring its significance in B cell function and immune regulation.

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