Invitrogen

MICA/B Monoclonal Antibody (6D4), PE, eBioscience™

Name: MICA\/B Monoclonal Antibody (6D4), PE, eBioscience\u2122
Brand: Invitrogen
SKU: 12-5788-42

2个已发表图片

5 篇参考文献

查看其他11个MICA/MICB抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
已发表图片 (2)

MICA/B Antibody in Flow Cytometry (Flow)

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MICA/B Antibody (12-5788-42) in Flow

HeLa cells were stained with Mouse IgG2a kappa Isotype Control, PE (Product #12-4724-82) (blue histogram) or MICA/B Monoclonal Antibody, PE (purple histogram). Total cells were used for analysis.

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产品信息

12-5788-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

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产品规格

种属反应

Human

已发表种属

C. elegans, Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

6D4

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10854117

产品详细信息

Description: The 6D4 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I chain-related (MIC), MICA and MICB proteins. MICA and MICB are related proteins of 83% amino acid similarity, and show homology with classical human leukocyte antigen (HLA) molecules. The structure of MICA and MICB are similar to classical HLA class I chains, however they do not bind beta2 microglobulin or bind peptide typical of HLA class I. MICA and MICB are expressed on the cell surface of endothelial cells, fibroblasts, gastric epithelium and PHA-stimulated T cells, and act as a ligand for NKGD2 expressed on the surface of NK cells, gammadelta T cells and alpha beta CD8+ T cells. There is evidence to suggest that human cytomegalovirus (HCMV) subverts NK cell detection by inhibiting the function of MICB. Furthermore, MICA and MICB expression has been detected in several epithelial tumours isolated from breast, lung, ovary, prostate, colon and kidney.

Applications Reported: This 6D4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 6D4 antibody has been pre-titrated and tested by flow cytometric analysis of HeLa cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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生物信息学

蛋白别名: HLA class I antigen; MHC class I chain-related protein A; MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I polypeptide-related sequence A; MHC class I polypeptide-related sequence B; MHC class I related sequence A; MHC class I-like molecule PERB11.2-IMX; MIC-A; MIC-B; MICA; MICB; sMICA; sMICB; soluble MICA; soluble MICB; stress inducible class I homolog; truncated MHC class I polypeptide-related sequence A

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基因别名: MIC-A; MICA; MICB; PERB11.1; PERB11.2

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UniProt ID: (Human) Q9GJ07, (Human) Q29980

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Entrez Gene ID: (Human) 100507436, (Human) 4277

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功能

antigen binding protein binding beta-2-microglobulin binding natural killer cell lectin-like receptor binding major histocompatibility complex protein

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