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Invitrogen
MICA/B Monoclonal Antibody (6D4), eBioscience™
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MICA/B Antibody (14-5788-82) in Flow
Staining of HeLa cell line with 0.25 µg of Mouse IgG2a kappa Isotype Control Purified (Product # 14-4724-82) (open histogram) or 0.25 µg of Anti-Human MICA/B Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Total viable cells were used for analysis.
产品信息
14-5788-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
6D4
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The 6D4 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I chain-related (MIC), MICA and MICB proteins. MICA and MICB are related proteins of 83% amino acid similarity, and show homology with classical human leukocyte antigen (HLA) molecules. The structure of MICA and MICB are similar to classical HLA class I chains, however they do not bind beta2 microglobulin or bind peptide typical of HLA class I. MICA and MICB are expressed on the cell surface of endothelial cells, fibroblasts, gastric epithelium and PHA-stimulated T cells, and act as a ligand for NKGD2 expressed on the surface of NK cells, gammadelta T cells and alpha beta CD8+ T cells. There is evidence to suggest that human cytomegalovirus (HCMV) subverts NK cell detection by inhibiting the function of MICB. Furthermore, MICA and MICB expression has been detected in several epithelial tumours isolated from breast, lung, ovary, prostate, colon and kidney.
Applications Reported: This 6D4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. (Please use Functional Grade purified 6D4, Product # 16-5788, in functional assays).
Applications Tested: This 6D4 antibody has been tested by flow cytometric analysis of HeLa cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: HLA class I antigen; MHC class I chain-related protein A; MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I polypeptide-related sequence A; MHC class I polypeptide-related sequence B; MHC class I related sequence A; MHC class I-like molecule PERB11.2-IMX; MIC-A; MIC-B; MICA; MICB; sMICA; sMICB; soluble MICA; soluble MICB; stress inducible class I homolog; truncated MHC class I polypeptide-related sequence A
基因别名: MIC-A; MICA; MICB; PERB11.1; PERB11.2
UniProt ID: (Human) Q9GJ07, (Human) Q29980
Entrez Gene ID: (Human) 100507436, (Human) 4277
T cell mediated cytotoxicity
immune response to tumor cell
cellular response to DNA damage stimulus
response to heat
cytolysis
antigen processing and presentation
negative regulation of natural killer cell activation
natural killer cell mediated cytotoxicity
defense response to bacterium
negative regulation of natural killer cell mediated cytotoxicity
gamma-delta T cell activation
regulation of immune response
defense response to virus
immune response-activating cell surface receptor signaling pathway
response to oxidative stress
viral process
response to retinoic acid
negative regulation of defense response to virus by host
