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Invitrogen
MICAL1 Polyclonal Antibody
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MICAL1 Antibody (PA5-90518) in WB
Western blot analysis of MICAL1 in extracts of various cell lines. Samples were incubated with MICAL1 Polyclonal antibody (Product # PA5-90518) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-90518
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein (or fragment)
偶联物
形式
Liquid
浓度
1.75 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: HRSCFRCHTC EATLWPGGYE QHPGDGHFYC LQHLPQTDHK AEGSDRGPES PELPTPSENS MPPGLSTPTA SQEGAGPVPD PSQPTRRQIR LSSPERQRLS SLNLTPDPEM EPPPKPPRSC SALARHALES SFVGWGLPVQ SPQALVAMEK EEKESPFSSE EEEEDVPLDS DVEQALQTFA KTSGTMNNYP TWRRTLLRRA KEEEMKRFCK AQTIQRRLNE IEAALRELEA EGVKLELALR RQSSSPEQQK KLWVGQLLQL VDKKNSLVAE EAELMITVQE LNLEEKQWQL DQELRGYMNR EENLKTAADR QAEDQVLRKL VDLVNQRDAL IRFQEERRLS ELALGTGAQG; Positive Samples: A-549, HT-29, Raji; Cellular Location: Cytoplasm, cytoskeleton
靶标信息
May be a cytoskeletal regulator that connects NEDD9 to intermediate filaments.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: [F-actin]-monooxygenase MICAL1; CasL interacting molecule MICAL; MICAL-1; microtubule associated monoxygenase, calponin and LIM domain containing 1; mMical1; Molecule interacting with CasL protein 1; NEDD9 interacting protein with calponin homology and LIM domains; NEDD9-interacting protein with calponin homology and LIM domains; protein-methionine sulfoxide oxidase MICAL1
基因别名: MICAL; MICAL-1; MICAL1; NICAL
UniProt ID: (Human) Q8TDZ2, (Mouse) Q8VDP3
Entrez Gene ID: (Human) 64780, (Mouse) 171580
actin binding
protein binding
zinc ion binding
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
SH3 domain binding
Rab GTPase binding
FAD binding
monooxygenase activity
3-(3-hydroxyphenyl)propionate hydroxylase activity
oxidoreductase activity
4-chlorobenzaldehyde oxidase activity
3,5-xylenol methylhydroxylase activity
phenylacetate hydroxylase activity
4-nitrophenol 4-monooxygenase activity
dimethyl sulfide monooxygenase activity
alpha-pinene monooxygenase [NADH] activity
phenanthrene 9,10-monooxygenase activity
1-hydroxy-2-naphthoate hydroxylase activity
toluene 4-monooxygenase activity
xylene monooxygenase activity
dibenzothiophene monooxygenase activity
6-hydroxy-3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity
chlorophenol 4-monooxygenase activity
carbon disulfide oxygenase activity
toluene 2-monooxygenase activity
1-hydroxy-2-oxolimonene 1,2-monooxygenase activity
phenanthrene 1,2-monooxygenase activity
tetrahydrofuran hydroxylase activity
styrene monooxygenase activity
toluene-4-sulfonate monooxygenase activity
toluene-sulfonate methyl-monooxygenase activity
3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity
2-hydroxy-phenylacetate hydroxylase activity
2-oxo-delta3-4,5,5-trimethylcyclopentenylacetyl-CoA 1,2-monooxygenase activity
phenanthrene 3,4-monooxygenase activity
toluene 3-monooxygenase activity
4-hydroxyphenylacetate,NADH:oxygen oxidoreductase (3-hydroxylating) activity
limonene monooxygenase activity
2-methylnaphthalene hydroxylase activity
1-methylnaphthalene hydroxylase activity
bisphenol A hydroxylase A activity
salicylate 5-hydroxylase activity
isobutylamine N-hydroxylase activity
branched-chain dodecylbenzene sulfonate monooxygenase activity
3-HSA hydroxylase activity
4-hydroxypyridine-3-hydroxylase activity
2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase activity
6-hydroxynicotinate 3-monooxygenase activity
metal ion binding
tocotrienol omega-hydroxylase activity
thalianol hydroxylase activity
actin or actin-binding cytoskeletal protein
negative regulation of protein phosphorylation
cytoskeleton organization
signal transduction
sulfur oxidation
actin filament depolymerization
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
oxidation-reduction process
negative regulation of apoptotic process
