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Invitrogen
MIDN Polyclonal Antibody
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MIDN Antibody (PA5-50521) in WB
Western Blot analysis of MIDN was performed using Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Stomach tissue lysates Lane 3: Rat Cerebrum tissue lysates. The membrane was probed with MIDN Polyclonal Antibody (Product # PA5-50521) at 1:1,000 dilution. Secondary antibody: at 1:20,000 dilution. Predicted band size: 49 kDa. Observed band size: 49 kDa.
产品信息
PA5-50521
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues near the N terminal of human Midnolin
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 50% glycerol
内含物
0.02% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
The antibody detects endogenous levels of total MIDN protein.
靶标信息
Expression of the midnolin gene is developmentally regulated: it is strongly expressed at the mesencephalon (midbrain) of the embryo in day 12.5 (E12.5) mice. The midnolin encodes a protein of 508 amino acids (aa), which contains a Ubiquitin-like domain. The intracellular distribution of the midnolin was studied by using midnolin-green fluorescent protein (GFP) fusion proteins. Midnolin was found to be localized in the nucleus and nucleolus, but not in the cytoplasm. The nucleolar localization signal was determined to be a 28aa peptide located at the C-terminal region of the midnolin. May be involved in regulation of genes related to neurogenesis in the nucleolus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DKFZp547M072; Midbrain nucleolar protein; Midnolin
基因别名: 3000003C15Rik; MIDN
UniProt ID: (Human) Q504T8, (Rat) D4AE48, (Mouse) Q3TPJ7
Entrez Gene ID: (Human) 90007, (Rat) 314623, (Mouse) 59090
