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AA Sequence of recombinant protein: ASNFDCCLGY TDRILHPKFI VGFTRQLANE GCDINAIIFH TKKKLSVCAN PKQTWVKYIV RLLSKKVKNM.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-3alpha (CCL20). Anti-Human MIP-3alpha (CCL20)-specific antibody was purified by affinity chromatography employing an immobilized Human MIP-3alpha (CCL20) matrix.
Sandwich ELISA: To detect Human MIP-3alpha (CCL20) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MIP-3alpha (CCL20) (500-P95ABt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3alpha (CCL20).
Western Blot: To detect HumanMIP-3alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIP-3alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Macrophage Inflammatory Protein-3 alpha (MIP-3alpha), also called CCL20, is expressed in the liver, lungs, lymph nodes and peripheral blood lymphocytes. MIP-3alpha is strongly up regulated by inflammatory signals, and down regulated by the anti-inflammatory cytokine IL-10. MIP3alpha signals through the G protein-coupled receptor, CCR6, and acts as a chemoattractant to lymphocytes and dendritic cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: beta chemokine exodus-1; Beta-chemokine exodus-1; C-C motif chemokine 20; CC chemokine LARC; chemokine (C-C motif) ligand 20; Liver and activation-regulated chemokine; M-MIP-3-alpha; Macrophage inflammatory protein 3 alpha; MIP 3 a; MIP 3 α; MIP 3alpha; MIP 3α; MIP-3-alpha; MIP3 a; MIP3 alpha; MIP3 α; small inducible cytokine subfamily A (Cys-Cys), member 20; Small-inducible cytokine A20
基因别名: CCL20; CKb4; Exodus; LARC; MIP-3-alpha; MIP-3a; MIP3A; SCYA20; ST38
UniProt ID: (Human) P78556
Entrez Gene ID: (Human) 6364