Invitrogen

MIP-3 beta Polyclonal Antibody, PeproTech®

Name: MIP-3 beta Polyclonal Antibody, PeproTech\u00AE
Brand: Invitrogen
SKU: 500-P95B-1MG

3 篇参考文献

查看其他13个CCL19抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

MIP-3 beta Antibody in Western Blot (WB)

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MIP-3 beta Antibody (500-P95B-1MG) in WB

Western Blot: To detect Human MIP-3beta by Western Blot analysis MIP-3 beta Polyclonal Antibody (Product # 500-P95B-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIP-3beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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Immunogen Image MIP-3 beta Polyclonal Antibody, PeproTech®

产品信息

500-P95B-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

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免疫染色 (IS)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human MIP-3beta (CCL19)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2930882

产品详细信息

AA Sequence of recombinant protein: GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPWVER IIQRLQRTSA KMKRRSS.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-3beta (CCL19). Anti-Human MIP-3beta (CCL19)-specific antibody was purified by affinity chromatography employing an immobilized Human MIP-3beta (CCL19) matrix.

Sandwich ELISA: To detect Human MIP-3beta (CCL19) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MIP-3beta (CCL19) (500-P95BBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3beta (CCL19).

Western Blot: To detect Human MIP-3beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIP-3beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

CCL19, also known as MIP-3 beta (Macrophage Inflammatory Protein 3 beta), is a member of the CC- subfamily of chemokines. It is most closely related to CCL21, with which it shares 32% amino acid sequence homology. CCL19 and CCL21 are expressed mainly by stromal cells in the T cell-rich zones of lymph nodes. They are critical mediators of the homeostatic trafficking of naive T cells and activated dendritic cells into the secondary lymphoid organs. CCL19 and CCL21 also play a role in T cell priming and activation, as well as the recruitment of lymphocytes to inflamed tissue. Both proteins signal via the G protein-coupled receptor, CCR7, which is expressed on T cells and mature dendritic cells. Although CCL19 and CCL21 both exhibit the same affinity for CCR7, only the binding of CCL19 results in the desensitization and internalization of the receptor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: beta chemokine exodus-3; Beta-chemokine exodus-3; C-C motif chemokine; C-C motif chemokine 19; CC chemokine ligand 19; chemokine (C-C motif) ligand 19; CK beta-11; EBI1 ligand chemokine; EBI1-ligand chemokine; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; M-MIP-3-beta; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MIP-3-beta; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19

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基因别名: CCL19; CKb11; ELC; MIP-3b; MIP3B; SCYA19

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UniProt ID: (Human) Q99731

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Entrez Gene ID: (Human) 6363

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功能

chemokine activity CCR7 chemokine receptor binding CCR10 chemokine receptor binding chemokine receptor binding CCR chemokine receptor binding cytokine intercellular signal molecule

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