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MKK2/Mitogen-activated protein kinase kinase 2 Polyclonal Antibody
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MKK2/Mitogen-activated protein kinase kinase 2 Antibody (AS15-2905) in WB
Optical densities (OD 600) of IPTG-induced E. coli expressing GST-MKK 2/MKK 4/MKK 5 were determined and sample volumes based on the equation 1/OD 600 were centrifuged to pellet bacteria. The resulting pellets were resuspended in 7 M urea, mixed with standard LaemmLi sample buffer and then the samples were denatured at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1 h onto NCL membrane u... View More
产品信息
AS15-2905
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana sequence of MKK2.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana (protoplasts)
靶标信息
MKK2 encodes a dual-specificity protein kinase that functions in the canonical RAS-RAF-MAP2K-ERK signaling module; the protein is predominantly cytoplasmic at steady state and acts as an upstream activator of ERK family MAP kinases. Structurally, MKK2 is a MAP kinase kinase with an N-terminal regulatory region mediating pathway-specific interactions and control, and a C-terminal conserved catalytic kinase domain that binds ATP and catalyzes phosphorylation on both threonine and tyrosine within the Thr-Glu-Tyr activation motif of downstream ERK MAP kinases. Biologically, MKK2 is activated by phosphorylation by MAP kinase kinase kinases in response to mitogens and growth factors, and in turn phosphorylates and activates ERK signaling to regulate core cellular programs including proliferation, differentiation, and survival; consistent with pathway relevance in development, pathogenic variants in MKK2 are implicated in cardiofaciocutaneous syndrome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ATMKK2; F27B13.50; F27B13_50; MAP KINASE KINASE 1; MAP2K; MAP2K2; MEK2; MK1
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