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Invitrogen
MKP-1 Polyclonal Antibody
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MKP-1 Antibody (PA5-85131) in WB
Western blot analysis of MKP-1 in mouse tissue extract using MKP-1 polyclonal antibody (Product # PA5-85131) using 50 µg of sample at a dilution of 1:200. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
产品信息
PA5-85131
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the center region of human DUSP1. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Keep as concentrated solution.
Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (92%), Xenopus laevis (95%), Pig (100%), Chicken (97%), Rhesus Monkey (100%), Bovine (100%).
Positive Control: mouse liver.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CL 100; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1; EC 3.1.3.16; EC 3.1.3.48; MAP kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 1; mitogen-activated protein kinase phosphatase-1; protein tyrosine phosphatase, non-receptor type 16; Protein-tyrosine phosphatase 3CH134; Protein-tyrosine phosphatase CL100; Protein-tyrosine phosphatase ERP; serine/threonine specific protein phosphatase
基因别名: 3CH134; CL100; DUSP1; erp; HVH1; MKP-1; MKP1; PTPN10; Ptpn16; U19515; VH1
UniProt ID: (Human) P28562, (Mouse) P28563
Entrez Gene ID: (Human) 1843, (Mouse) 19252
non-membrane spanning protein tyrosine phosphatase activity
protein binding
protein tyrosine/threonine phosphatase activity
MAP kinase tyrosine/serine/threonine phosphatase activity
growth factor binding
phosphoprotein phosphatase activity
protein tyrosine phosphatase activity
protein tyrosine/serine/threonine phosphatase activity
hydrolase activity
phosphatase activity
protein phosphatase
inactivation of MAPK activity
endoderm formation
response to oxidative stress
response to light stimulus
response to estradiol
response to retinoic acid
cellular response to hormone stimulus
response to testosterone
peptidyl-tyrosine dephosphorylation
intracellular signal transduction
peptidyl-threonine dephosphorylation
response to hydrogen peroxide
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of MAP kinase activity
negative regulation of MAPK cascade
response to glucocorticoid
negative regulation of meiotic cell cycle
response to cAMP
response to calcium ion
negative regulation of ERK1 and ERK2 cascade
mitotic cell cycle arrest
regulation of mitotic cell cycle spindle assembly checkpoint
negative regulation of DNA biosynthetic process
protein dephosphorylation
cell cycle
dephosphorylation
