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MOV10L1 Antibody (PA5-98898) in ICC/IF
Immunofluorescent analysis of MOV10L1 in HepG2 cells using a MOV10L1 polyclonal antibody (Product # PA5-98898) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.
产品信息
PA5-98898
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human RNA helicase Mov10l1 protein (336-425AA)
偶联物
形式
Liquid
浓度
4.06 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% ProClin 300
保存条件
-20°C or -80°C if preferred
运输条件
Wet ice
RRID
AB_2813511
靶标信息
Isoform 1: ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for germline integrity (PubMed:20534472, PubMed:20547853, PubMed:23166510, PubMed:25762440). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:20534472, PubMed:20547853, PubMed:23166510, PubMed:25762440). Involved in the primary piRNA metabolic process (PubMed:20534472, PubMed:20547853, PubMed:23166510, PubMed:25762440). Specifically binds to piRNA precursors and promotes the generation of intermediate piRNA processing fragments that are subsequently loaded to Piwi proteins (PubMed:25762440). Acts via its ATP-dependent RNA helicase activity: displays 5'-3' RNA unwinding activity and probably mediates unwinding and funneling of single-stranded piRNA precursor transcripts to the endonuclease that catalyzes the first cleavage step of piRNA processing to generate piRNA intermediate fragments that are subsequently loaded to Piwi proteins (PubMed:25762440). {ECO:0000269; PubMed:20534472, ECO:0000269; PubMed:20547853, ECO:0000269; PubMed:23166510, ECO:0000269; PubMed:25762440}.; Isoform 2: May act downstream of MEF2C during heart formation. Acts as a cardiac-specific suppressor of cardiomyocyte hypertrophy and cell cycle progression, suggesting that it may suppress these processes through the regulation of CDKN1A. Such results however require additional evidence. {ECO:0000305; PubMed:11854500}.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cardiac helicase activated by MEF2C protein; Moloney leukemia virus 10-like protein 1; Mov10-like 1; MOV10-like protein 1; Mov10l1, Moloney leukemia virus 10-like 1, homolog; putative helicase Mov10l1; RNA helicase Mov10l1
基因别名: CHAMP; DJ402G11.8; MOV10L1
UniProt ID: (Human) Q9BXT6
Entrez Gene ID: (Human) 54456
