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Invitrogen
MPI Polyclonal Antibody
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MPI Antibody (PA5-96635) in WB
Western blot analysis of MPI in extracts of various cell lines. Samples were incubated with MPI Polyclonal antibody (Product # PA5-96635) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
PA5-96635
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-423 of human MPI (NP_0024261)
偶联物
形式
Liquid
浓度
1.55 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: MAAPRVFPLS CAVQQYAWGK MGSNSEVARL LASSDPLAQI AEDKPYAELW MGTHPRGDAK ILDNRISQKT LSQWIAENQD SLGSKVKDTF NGNLPFLFKV LSVETPLSIQ AHPNKELAEK LHLQAPQHYP DANHKPEMAI ALTPFQGLCG FRPVEEIVTF LKKVPEFQFL IGDEAATHLK QTMSHDSQAV ASSLQSCFSH LMKSEKKVVV EQLNLLVKRI SQQAAAGNNM EDIFGELLLQ LHQQYPGDIG CFAIYFLNLL TLKPGEAMFL EANVPHAYLK GDCVECMACS DNTVRAGLTP KFIDVPTLCE MLSYTPSSSK DRLFLPTRSQ EDPYLSIYDP PVPDFTIMKT EVPGSVTEYK VLALDSASIL LMVQGTVIAS TPTTQTPIPL QRGGVLFIGA NESVSLKLTE PKDLLIFRAC CLL; Positive Samples: Jurkat, HepG2, HeLa, SW620, Mouse heart; Cellular Location: Cytoplasm
靶标信息
Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: mannose phosphate isomerase 1; Mannose-6-phosphate isomerase; Phosphohexomutase; Phosphomannose isomerase; phosphomannose isomerase 1; PMI
基因别名: 1110002E17Rik; AI315153; CDG1B; MPI; Mpi-1; Mpi1; PMI; PMI1
UniProt ID: (Human) P34949, (Rat) Q68FX1, (Mouse) Q924M7
Entrez Gene ID: (Human) 4351, (Rat) 300741, (Mouse) 110119
