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Invitrogen
MPS1 Polyclonal Antibody
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MPS1 Antibody (PA5-79936) in ICC/IF
Immunocytochemistry analysis of MPS1 using anti-MPS1 antibody (Product # PA5-79936) . MPS1 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-MPS1 antibody (Product # PA5-79936) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visua... View More
产品信息
PA5-79936
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived human MPS1 recombinant protein (Position: P2-H84).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Caco-2 whole cell, human RT4 whole cell, human Jurkat whole cell, rat PC-12 whole cell, rat RH35 whole cell, mouse RAW2647 whole cell, mouse SP2/0 whole cell. IHC: human intestinal cancer tissue, mouse brain cancer tissue, rat brain cancer tissue. ICC/IF: U20S cell. Flow: HeLa cell.
靶标信息
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 40S ribosomal protein S27; Metallopan-stimulin 1; metallopanstimulin 1; MPS-1; RP11-422P24.3; rps27; rps27 {ECO:0000312|RGD:621045}; Small ribosomal subunit protein eS27
基因别名: 3200001M24Rik; MPS-1; MPS1; RPS27; S27; S27-1
UniProt ID: (Human) P42677, (Mouse) Q6ZWU9, (Rat) Q71TY3
Entrez Gene ID: (Human) 6232, (Mouse) 57294, (Rat) 94266
