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Invitrogen
MRPL2 Polyclonal Antibody
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MRPL2 Antibody (PA5-89805) in WB
Western blot analysis of MRPL2 in extracts of various cell lines. Samples were incubated with MRPL2 Polyclonal antibody (Product # PA5-89805) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-89805
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human MRPL2 (NP_0570342)
偶联物
形式
Liquid
浓度
1.54 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: MALCALTRAL RSLNLAPPTV AAPAPSLFPA AQMMNNGLLQ QPSALMLLPC RPVLTSVALN ANFVSWKSRT KYTITPVKMR KSGGRDHTGR IRVHGIGGGH KQRYRMIDFL RFRPEETKSG PFEEKVIQVR YDPCRSADIA LVAGGSRKRW IIATENMQAG DTILNSNHIG RMAVAAREGD AHPLGALPVG TLINNVESEP GRGAQYIRAA GTCGVLLRKV NGTAIIQLPS KRQMQVLETC VATVGRVSNV DHNKRVIGKA GRNRWLGKRP NSGRWHRKGG WAGRKIRPLP PMKSYVKLPS ASAQS; Positive Samples: Mouse kidney, Mouse lung; Cellular Location: Mitochondrion
靶标信息
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 12q. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 39S ribosomal protein L2, mitochondrial; L2mt; Large ribosomal subunit protein uL2m; MRP-L2; ribosomal protein, mitochondrial, L14
基因别名: CGI-22; MRP-L14; MRPL2; RPML14
UniProt ID: (Human) Q5T653, (Mouse) Q9D773
Entrez Gene ID: (Human) 51069, (Mouse) 27398
