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Agrisera
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Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Nicotiana tabacum
Malate synthase is a soluble, cytosolic enzyme of the glyoxylate pathway that is generally encoded in bacteria and fungi (and, in plants, functions within the peroxisome-derived glyoxysome compartment), where it supports net assimilation of C2 units by bypassing the CO2-releasing steps of the tricarboxylic acid cycle. Structural studies of the canonical bacterial malate synthase G show it is a ~80 kDa monomeric enzyme built around a central beta8/alpha8 TIM-barrel core with additional N-terminal and inserted alpha/beta domains and a C-terminal helical "plug" that helps cap the active-site cleft; catalysis requires a divalent metal, typically Mg2+, coordinated with the aldehyde/carboxylate functions of glyoxylate in the active site. Functionally, malate synthase catalyzes the Mg2+-dependent Claisen-like condensation of glyoxylate with acetyl-CoA followed by hydrolysis of the thioester intermediate to yield (S)-malate and free coenzyme A, thereby replenishing C4 dicarboxylic acids for biosynthesis and gluconeogenic flux during growth on acetate, fatty acids, or other nonfermentable carbon sources, and it is frequently important for metabolic persistence and virulence in several pathogens.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: aceB; AoMls; DAL7; glcB; MLS; MLS1