Invitrogen

MSH2 Monoclonal Antibody (SP46)

查看其他44个MSH2抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (2)

MSH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MSH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MSH2 Antibody (MA5-16379) in IHC (P)

Immunohistochemical (paraffin) analysis of MSH2 using anti-MSH2 Monoclonal Antibody (Product # MA5-16379) in Colon Carcinoma Cancer Tissue. The recommended dilution for this antibody in immunohistochemistry applications is 1:50.

MSH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MSH2 Antibody in Western Blot (WB)

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MSH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical (paraffin) analysis of MSH2 using anti-MSH2 Monoclonal Antibody (Product # MA5-16379) in Colon Carcinoma Cancer Tissue. The recommended dilution for this antibody in immunohistochemistry applications is 1:50.

MSH2 Antibody in Western Blot (WB) — Western blot was performed using Anti-MSH2 Monoclonal Antibody (SP46)(Product # MA5-16379) and a 105kDa band corresponding to MSH2 was observed across cell lines tested. Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1) and HEK-293 (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

产品信息

MA5-16379

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫组化（石蜡） (IHC (P))

1:50

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

SP46

抗原

Synthetic peptide corresponding to internal region of human MSH2.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Protein A/G

保存液

PBS, pH 7.2, with 1% BSA

内含物

0.1% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is colon carcinoma.

靶标信息

Plays a role in neurogenesis and neuronal migration. Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: DNA mismatch repair protein Msh2; hMSH2; Msh2p; mutS homolog 2, colon cancer, nonpolyposis type 1; MutS protein homolog 2

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基因别名: COCA1; FCC1; HNPCC; HNPCC1; LCFS2; MSH2

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UniProt ID: (Human) P43246

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Entrez Gene ID: (Human) 4436

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magnesium ion binding four-way junction DNA binding Y-form DNA binding heteroduplex DNA loop binding double-strand/single-strand DNA junction binding DNA binding double-stranded DNA binding single-stranded DNA binding protein binding ATP binding protein C-terminus binding ATPase activity centromeric DNA binding enzyme binding protein kinase binding mismatched DNA binding guanine/thymine mispair binding dinucleotide insertion or deletion binding single guanine insertion binding single thymine insertion binding dinucleotide repeat insertion binding oxidized purine DNA binding MutLalpha complex binding protein homodimerization activity ADP binding DNA metabolism protein

meiotic mismatch repair in utero embryonic development oxidative phosphorylation DNA repair mismatch repair postreplication repair double-strand break repair meiotic gene conversion cell cycle arrest reciprocal meiotic recombination germ cell development determination of adult lifespan male gonad development response to X-ray response to UV-B somatic hypermutation of immunoglobulin genes somatic recombination of immunoglobulin gene segments B cell mediated immunity B cell differentiation intra-S DNA damage checkpoint intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator negative regulation of neuron apoptotic process maintenance of DNA repeat elements negative regulation of reciprocal meiotic recombination isotype switching negative regulation of DNA recombination positive regulation of helicase activity