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Invitrogen
MSH3 Polyclonal Antibody
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FIGURE: 1 / 4
MSH3 Antibody (PA5-29829) in ICC/IF
MSH3 Polyclonal Antibody detects MSH3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MSH3 stained by MSH3 Polyclonal Antibody (Product # PA5-29829) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
Product Details
PA5-29829
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human MSH3. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.79 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: A549, H1299.
Predicted reactivity: Human (99%), Mouse (83%), Rat (85%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Divergent upstream protein; DNA mismatch repair protein Msh3; DUP; hMSH3; Mismatch repair protein 1; MRP1
Gene Aliases: DUC1; DUG; DUP; MRP1; MSH3
UniProt ID: (Human) P20585
Entrez Gene ID: (Human) 4437
damaged DNA binding
single-stranded DNA binding
protein binding
ATP binding
enzyme binding
mismatched DNA binding
guanine/thymine mispair binding
dinucleotide insertion or deletion binding
single guanine insertion binding
dinucleotide repeat insertion binding
oxidized purine DNA binding
protein homodimerization activity
DNA metabolism protein
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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