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Invitrogen
MTCO1 Monoclonal Antibody (1D6E1A8)
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MTCO1 Antibody (459600) in ICC/IF
Immunofluorescence analysis of MTCO1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MTCO1 Monoclonal Antibody (1D6E1A8) (Product # 459600) at 5 µg/mL dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Produc... View More
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.jpg?time=20220826?time=20220826 "MTCO1 Antibody (459600) in WB")
产品信息
459600
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Arthropod,
C. elegans,
Guinea pig,
Human,
Mouse,
Rat,
Zebrafish
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
1D6E1A8
抗原
Human Mitochondrial Complex IV subunit I
偶联物
形式
Liquid
浓度
0.99 mg/mL
纯化类型
Protein A
保存液
PBS with 0.05% BSA, 40% glycerol
内含物
0.01% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
For best results with this antibody in Western blot, do not boil samples before loading onto the gel. Boiling of the sample will cause a loss of signal. Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio. In Western blot applications, this antibody detects a band at approximately 40 kDa (predicted MW: 57 kDa). In mouse liver lysate a specific band below 37 kDa was detected.
Recommended positive controls:
ICC/IF - Pig retinal pigment epithelial cells, rat cerebellum primary cells
IHC (P) - Human skeletal muscle and colon tissue, rat pancreas tissue, pig smooth muscle tissue
WB - Mouse, human, bovine and rat heart mitochondria lysate
Flow - HEK-293 cells
Recommended long term storage at -20°C.
靶标信息
Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al. (2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant; cultured cells can maintain several mtDNA haplotypes at stable frequencies; the respiratory chain has little spare COX capacity; and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Co1; Cytochrome c oxidase polypeptide I; Cytochrome c oxidase subunit 1
基因别名: COI; COXI; MT-CO1; MTCO1
UniProt ID: (Mouse) P00397
Entrez Gene ID: (Mouse) 17708
