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Invitrogen
MTCO1 Recombinant Rabbit Monoclonal Antibody (PSH07-50)
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MTCO1 Antibody (MA5-60181) in ICC/IF
Immunofluorescence analysis of MTCO1 using Neuro-2a. The cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. The cells were labeled with MTCO1 Recombinant Rabbit Monoclonal Antibody (PSH07-50) (Product # MA5-60181, green) and beta tubulin (red) at 1:50 and 1:100 dilution, respectively, in 1% BSA in PBST overnight at 4 degrees Celsius and then labeled with ... View More
产品信息
MA5-60181
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PSH07-50
抗原
Recombinant protein within human MTCO1.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Dry ice (international)
RRID
产品详细信息
Positive Control: HeLa cell lysate, MCF7 cell lysate, Huh7 cell lysate, COLO205 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, C6 cell lysate, PC-12 cell lysate, Neuro-2a, C6, human colon tissue, human kidney tissue, mouse colon tissue, mouse kidney tissue, rat colon tissue, rat kidney tissue, HeLa.
Tissue Specificity: Tissue enhanced (heart muscle, tongue).
Subcellular Location: Mitochondrion inner membrane.
Sequence Similarities: 75% Mouse/Rat.
Predicted band size: 57 kDa.
靶标信息
Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al. (2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant; cultured cells can maintain several mtDNA haplotypes at stable frequencies; the respiratory chain has little spare COX capacity; and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Co1; Cytochrome c oxidase polypeptide I; Cytochrome c oxidase subunit 1
基因别名: COI; COXI; MT-CO1; MTCO1
UniProt ID: (Mouse) P00397
Entrez Gene ID: (Mouse) 17708
