Invitrogen

MVP Monoclonal Antibody (8B12)

Name: MVP Monoclonal Antibody (8B12)
Brand: Invitrogen
SKU: MA5-49162

查看其他29个MVP抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (10)

MVP Antibody in Immunocytochemistry (ICC/IF)

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MVP Antibody (MA5-49162) in ICC/IF

Immunocytochemistry analysis of MVP in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in MVP Monoclonal antibody (Product # MA5-49162) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the... View More

MVP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image MVP Monoclonal Antibody (8B12)

产品信息

MA5-49162

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

8B12

抗原

E.coli-derived human MVP recombinant protein (Position: A2-H259).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_3074820

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human A549 tissue, human U2OS whole cell, human HepG2 whole cell, human THP-1 whole cell, human Hela whole cell, human SW620 whole cellrat RH35 whole cellmouse RAW2467 whole cell. IHC: mouse intestine tissue, human colon cancer tissue, rat intestine tissue, human mammary cancer tissue, human Lung cancer tissue, human Lung cancer tissue. ICC/IF: A431 cell. Flow: A549 cell, A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

This gene encodes the major vault protein which is a lung resistance-related protein. Vaults are multi-subunit structures that may be involved in nucleo-cytoplasmic transport. This protein mediates drug resistance, perhaps via a transport process. It is widely distributed in normal tissues, and overexpressed in multidrug-resistant cancer cells. The protein overexpression is a potentially useful marker of clinical drug resistance. This gene produces two transcripts by using two alternative exon 2 sequences; however, the open reading frames are the same in both transcripts.

仅用于科研。不用于诊断过程。未经明确授权不得转售。