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Invitrogen
MXD1 Polyclonal Antibody
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MXD1 Antibody (PA5-79711) in WB
Western blot analysis of Mad in Lane 1: rat testis tissue lysate, Lane 2: mouse ovary tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with Mad polyclonal antibody (Product # PA5-79711) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat an... View More
产品信息
PA5-79711
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E. coli-derived human Mad recombinant protein (Position: M1-L221).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat testis tissue, mouse ovary tissue.
靶标信息
MAX dimerization protein belongs to a subfamily of MAX-interacting proteins. This protein competes with MYC for binding to MAX to form a sequence-specific DNA-binding complex, acts as a transcriptional repressor (while MYC appears to function as an activator) and is a candidate tumor suppressor.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: antagonizer of myc transcriptional activity; Max dimerization protein 1; Max dimerizer 1; MAX-binding protein; MGC104659; OTTHUMP00000202211; Protein MAD
基因别名: AW122478; BHLHC58; MAD; Mad-1; MAD1; MXD1
UniProt ID: (Human) Q05195
Entrez Gene ID: (Human) 4084, (Rat) 362391, (Mouse) 17119
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription cofactor activity
transcription corepressor activity
protein binding
protein dimerization activity
DNA binding
basic helix-loop-helix transcription factor
