Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
MYH1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 7
MYH1 Antibody (PA5-31466) in ICC/IF
Immunofluorescence analysis of Myosin Skeletal Muscle was performed using C2C12 and C2C12 cells differentiated to myotubes (2% Horse serum for 5 days). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Myosin Skeletal Muscle Rabbit Polyclonal Antibody (Product # PA5-31466) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Super... View More
Product Details
PA5-31466
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human MYH1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.18 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: RD, mouse heart.
Predicted reactivity: Mouse (98%), Rat (97%), Xenopus laevis (81%), Dog (98%), Pig (99%), Rabbit (97%), Rhesus Monkey (100%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Myosin is a protein which is known to interact with actin in muscle and non-muscle cells. It contains two identical heavy chains and four light chains. Myosin molecules consist of two major regions: tails (rods) and heads. They aggregate into filaments through the tail region and interact with actin and with ATP through the head region. Multiple forms of myosin heavy chains exist for each muscle type-skeletal, cardiac, smooth and non-muscle isomyosin forms exist in different types of skeletal muscle, depending on the physiological function of the muscle. They are designated at type I (slow twitch) and type II (fast twitch).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis luminal protein 71; MyHC-2x; MyHC-IIx/d; Myosin heavy chain 1; Myosin heavy chain 2x; Myosin heavy chain IIx/d; Myosin heavy chain, skeletal muscle, adult 1; myosin, heavy chain 1, skeletal muscle, adult; myosin, heavy polypeptide 1, skeletal muscle, adult; Myosin-1; type 2X myosin heavy chain
Gene Aliases: A530084A17Rik; HEL71; IId; IId/x; MdMs; MHC-2X/D; MHC2X/D; MYH1; MYHa; MYHC; MyHC-2x; MyHC-2X/D; MyHC-IId/x; MYHC-IIX; MyHC-IIx/d; Myhs-f; Myhs-f2; MYHSA1; Myhsf2
UniProt ID: (Human) P12882, (Mouse) Q5SX40
Entrez Gene ID: (Human) 4619, (Mouse) 17879, (Rat) 287408
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support