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Invitrogen
MYH9 Polyclonal Antibody
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FIGURE: 1 / 6
MYH9 Antibody (PA5-27506) in ICC/IF
MYH9 Polyclonal Antibody detects MYH9 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MYH9 stained by MYH9 Polyclonal Antibody (Product # PA5-27506) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.
Product Details
PA5-27506
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human MYH9. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.55 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, NT2D1, PC-3.
Predicted reactivity: Mouse (98%), Rat (97%), Xenopus laevis (92%), Dog (99%), Chicken (96%), Bovine (99%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The MYH9 gene, located on chromosome 22q12.3, encodes the heavy chain of non-muscle myosin IIA (NMHC IIA), a critical component of the actin cytoskeleton that plays essential roles in various cellular processes. Structurally, the MYH9 gene spans over 106 kilobases and includes 41 exons that translate into a protein of 1,960 amino acids. This protein is a part of a hexameric complex, which includes two heavy chains, two regulatory light chains, and two essential light chains. The NMHC IIA protein interacts with actin filaments and is involved in cellular activities such as cell migration, adhesion, division, and maintenance of cell shape. Functionally, mutations in MYH9 can result in a spectrum of autosomal dominant disorders collectively known as MYH9-related diseases (MYH9-RD), which include conditions such as May-Hegglin anomaly, Fechtner syndrome, and Epstein syndrome. These disorders are primarily characterized by macrothrombocytopenia (abnormally large platelets) and may lead to other complications such as hearing loss, renal failure, and cataracts later in life. MYH9 is also crucial in hematopoiesis, where its proper function is necessary for the survival and maintenance of hematopoietic stem and progenitor cells (HSPCs). Loss of MYH9 function disrupts normal hematopoiesis, leading to severe blood cell deficiencies and bone marrow failure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cellular myosin heavy chain, type A; MGC104539; Myosin heavy chain 9; Myosin heavy chain, non-muscle IIa; Myosin-9; myosin9; NMHC-II A; NMMHC II-a; NMMHC IIA; NMMHC-A; non-muscle myosin heavy chain 9; Non-muscle myosin heavy chain A; Non-muscle myosin heavy chain IIa; non-muscle myosin heavy polypeptide 9; nonmuscle myosin heavy chain II-A
Gene Aliases: BDPLT6; DFNA17; EPSTS; FTNS; MHA; MYH9; NMHC-II-A; NMMHC-IIA; NMMHCA
UniProt ID: (Human) P35579
Entrez Gene ID: (Human) 4627
microfilament motor activity
motor activity
actin binding
protein binding
calmodulin binding
ATP binding
ATPase activity
protein domain specific binding
actin-dependent ATPase activity
protein homodimerization activity
protein anchor
ADP binding
poly(A) RNA binding
actin filament binding
cadherin binding involved in cell-cell adhesion
meiotic spindle organization
cytokinesis
angiogenesis
in utero embryonic development
establishment of T cell polarity
membrane protein ectodomain proteolysis
phagocytosis, engulfment
integrin-mediated signaling pathway
myoblast fusion
regulation of cell shape
protein transport
actin filament-based movement
platelet formation
monocyte differentiation
actomyosin structure organization
actin cytoskeleton reorganization
uropod organization
blood vessel endothelial cell migration
leukocyte migration
establishment of meiotic spindle localization
platelet aggregation
negative regulation of actin filament severing
positive regulation of protein processing in phagocytic vesicle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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