Invitrogen

MYO7A Polyclonal Antibody

Name: MYO7A Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-936

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8个已发表图片

20 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

MYO7A Antibody in Immunocytochemistry (ICC/IF)

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MYO7A Antibody (PA1-936) in ICC/IF

Immunofluorescence analysis of MYO7A was performed using 70% confluent log phase L6 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with MYO7A Rabbit Polyclonal Antibody (Product # PA1-936) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A270... View More

MYO7A Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

MYO7A Antibody in Immunohistochemistry (IHC)

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MYO7A Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Relative expression of MYO7A was observed in Mouse Skeletal Muscle, Rat Skeletal Muscle, Mouse Bone and Rat Heart in Western Blot using MYO7A Polyclonal Antibody (Product # PA1-936). MYO7A is reported to be expressed in skeletal tissues and not in other tissues like bone and heart. {RE}

Immunogen image

Immunogen Image MYO7A Polyclonal Antibody

产品信息

PA1-936

应用

建议稀释比

已发表文章

免疫印迹 (WB)

4 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

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免疫组织化学（多聚甲醛固定） (IHC (PFA))

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免疫细胞化学 (ICC/IF)

2 µg/mL

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产品规格

种属反应

Mouse, Rat

已发表种属

C. elegans, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: S(16) G Q E F D V P I G A V V K L C(31)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2235704

产品详细信息

PA1-936 detects myosin VIIa from mouse tissues as well as recombinant protein.

PA1-936 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~220 kDa protein representing myosin VIIa from mouse testes preparations. PA1-936 detects recombinant mouse myosin VIIa overexpressed in Sf9 insect cell lysate.

PA1-936 immunizing peptide corresponds to amino acid residues 16-31 from mouse myosin VIIa. This sequence is completely conserved in mouse, porcine, and human myosin VIIa. PA1-936 immunizing peptide (Cat. # PEP-045) is available for use in neutralization and control experiments.

靶标信息

Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: motor protein; MyO VIIa; myosin 7a; myosin VIIA (Usher syndrome 1B (autosomal recessive, severe)); myosin-VIIa; shaker 1; Unconventional myosin-VIIa

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基因别名: Hdb; Myo7; Myo7a; nmf371; polka; sh-1; sh1; USH1B

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UniProt ID: (Mouse) P97479

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Entrez Gene ID: (Mouse) 17921, (Rat) 266714

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功能