Invitrogen

Menin Recombinant Rabbit Monoclonal Antibody (25GB2590)

查看其他21个Menin抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (4)

Menin Antibody in Immunocytochemistry (ICC/IF)

Menin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

Menin Antibody (MA570275) in ICC/IF

Immunocytochemistry analysis of Menin in HepG2 cells. Sample was incubated with Menin monoclonal antibody (Product # MA570275) at a dilution of 1:1,000. Nuclei were stained blue with DAPI and Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Menin Antibody in Immunocytochemistry (ICC/IF)

Menin Antibody in Western Blot (WB)

Menin Antibody in Western Blot (WB)

Menin Antibody in Flow Cytometry (Flow)

Product Image Gallery

Menin Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Menin in HepG2 cells. Sample was incubated with Menin monoclonal antibody (Product # MA570275) at a dilution of 1:1,000. Nuclei were stained blue with DAPI and Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Menin Antibody in Western Blot (WB) — Western blot analysis of Menin. Samples (HT-1080, HepG2, HeLa, H9C2, NIH/3T3) were incubated with Menin monoclonal antibody (Product # MA570275) at a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ ECL Substrate Kit.

Menin Antibody in Western Blot (WB) — Western blot analysis of Menin. Sample was incubated with Menin monoclonal antibody (Product # MA570275) at a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQECL Substrate Kit.

Menin Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of Menin in HepG2 cells. Sample was incubated with Menin monoclonal antibody (Product # MA570275) at a dilution of 1:2,000. Green, isotype control; red, Menin 1.

Immunogen image

Menin Recombinant Rabbit Monoclonal Antibody (25GB2590)

Immunogen Image Menin Recombinant Rabbit Monoclonal Antibody (25GB2590)

产品信息

MA570275

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

-

免疫细胞化学 (ICC/IF)

1:1,000

-

流式细胞分析 (Flow)

1:2,000

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293F

分类

Recombinant Monoclonal

类型

Antibody

克隆号

25GB2590

抗原

A synthesized peptide derived from human (550-610) Menin.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Dry ice (international)

靶标信息

Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Menin; multiple endocrine neoplasia 1; multiple endocrine neoplasia I

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基因别名: AW045611; MEAI; MEN1; menin; SCG2

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UniProt ID: (Human) O00255, (Mouse) O88559, (Rat) Q9WVR8

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Entrez Gene ID: (Human) 4221, (Mouse) 17283, (Rat) 29417

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four-way junction DNA binding Y-form DNA binding chromatin binding double-stranded DNA binding protein binding histone-lysine N-methyltransferase activity protein binding, bridging transcription regulatory region DNA binding protein N-terminus binding R-SMAD binding DNA binding sequence-specific DNA binding molecular_function

negative regulation of transcription from RNA polymerase II promoter MAPK cascade ossification leukocyte homeostasis negative regulation of protein phosphorylation osteoblast fate commitment osteoblast development type B pancreatic cell differentiation chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated cellular response to DNA damage stimulus cell cycle arrest negative regulation of cell proliferation response to UV embryo development response to gamma radiation negative regulation of cell-substrate adhesion chromatin modification histone methylation hemopoiesis positive regulation of transforming growth factor beta receptor signaling pathway positive regulation of histone methylation positive regulation of protein binding regulation of activin receptor signaling pathway histone lysine methylation positive regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of cell differentiation negative regulation of osteoblast differentiation positive regulation of osteoblast differentiation negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of JNK cascade negative regulation of organ growth embryonic skeletal system morphogenesis negative regulation of epithelial cell proliferation positive regulation of cell division negative regulation of telomerase activity palate development maternal process involved in female pregnancy regulation of type B pancreatic cell proliferation response to transforming growth factor beta negative regulation of cell cycle G1/S phase transition mitotic cell cycle brain development decidualization cellular response to glucose stimulus cellular response to peptide hormone stimulus DNA repair beta-catenin-TCF complex assembly