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Invitrogen
Metadherin Monoclonal Antibody (2F11C3)
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Metadherin Antibody (MA5-15564) in ICC/IF
Immunofluorescence analysis of MTDH was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Metadherin Monoclonal Antibody (2F11C3) (Product # MA5-15564, 1:100) in 0.1% BSA, incubated at 4 degree celsius overni... View More
产品信息
MA5-15564
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
2F11C3
抗原
Purified recombinant fragment of human Metadherin expressed in E. Coli.
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
ascites
内含物
0.03% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA5-15564 targets Metadherin in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15564 immunogen is purified recombinant fragment of human Metadherin expressed in E. Coli.
MA5-15564 detects Metadherin which has a predicted molecular weight of approximately 64kDa.
靶标信息
Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 3D3/LYRIC; AEG-1; astrocyte elevated gene 1; Astrocyte elevated gene-1 protein; LYRIC/ MTDH; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein; Protein LYRIC
基因别名: 3D3; AEG-1; AEG1; LYRIC; LYRIC/3D3; MTDH
UniProt ID: (Human) Q86UE4
Entrez Gene ID: (Human) 92140
negative regulation of transcription from RNA polymerase II promoter
positive regulation of autophagy
lipopolysaccharide-mediated signaling pathway
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of angiogenesis
positive regulation of NF-kappaB transcription factor activity
positive regulation of protein kinase B signaling
bicellular tight junction assembly
