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H4K20me1 Antibody (MA5-18067) in ICC/IF
Immunocytochemistry analysis of H4K20me1 in paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Samples were incubated with H4K20me1 monoclonal antibody (Product # MA5-18067) using a dilution of 1:100 for 1hr. Followed by Alexa Fluor® 488 secondary antibody at a 1:1,000 dilution, showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.
产品信息
MA5-18067
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
5E10-D8
抗原
Synthetic peptide within Human Histone H4 (mono methyl K20)(Immunogen sequence: 15-AKRHR(Kme)VLRDN-25 (branched))
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.1% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody is specific for K20 mono-methylated histone H4 and does not cross-react with histone 3 or tri-, di-, or unmethylated histone H4. This antibody is predicted to react with Xenopus laevis, Arabidopsis thaliana, Fruit fly (Drosophila melanogast), and most mammals based on sequence homology.
This target has a predicted molecular weight of 11 kDa. Actual Western blot results show a band at 13 kDa. This can be blocked using mono-methyl K20 Histone H4 peptide.
Click here for Master Lot linking information.
靶标信息
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: H4 histone family, member A; H4 histone family, member E; H4 histone family, member I; H4 histone family, member J; H4 histone family, member M; H4 histone family, member N; H4 histone, family 2; H4 K20; H4K20; H4K20me; H4Lys20me; H4Lys20me1; HIST1H4; histone 1, H4a; histone 1, H4b; histone 1, H4e; histone 1, H4i; histone 1, H4j; histone 2, H4a; Histone 4; Histone 4 family, member M; histone 4, H4; Histone Cluster 4; histone family member; Histone H4; Histone H4A; Histone H4K20me1; histone IV, family 2; methyl Histone 3; methyl Histone H3; Methyl-H4 K20; Methyl-H4 Lys20; Methyl-Histone H4 K20; Methyl-Histone H4 Lys20; Mono-methyl H4; Mono-methyl Histone H4
基因别名: dJ160A22.2; FO108; H4; H4-16; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4F2iv; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4
UniProt ID: (Human) P62805
Entrez Gene ID: (Human) 8359, (Human) 8366, (Human) 8367, (Human) 8294, (Human) 8363, (Human) 8370, (Human) 121504
chromatin silencing at rDNA
double-strand break repair via nonhomologous end joining
nucleosome assembly
DNA replication-dependent nucleosome assembly
DNA replication-independent nucleosome assembly
DNA-templated transcription, initiation
telomere capping
gene silencing by RNA
telomere organization
CENP-A containing nucleosome assembly
cellular protein metabolic process
negative regulation of megakaryocyte differentiation
negative regulation of gene expression, epigenetic
positive regulation of gene expression, epigenetic
protein heterotetramerization
beta-catenin-TCF complex assembly
