Invitrogen

Moesin Monoclonal Antibody (8D4)

Name: Moesin Monoclonal Antibody (8D4)
Brand: Invitrogen
SKU: MA5-49167

查看其他30个Moesin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (8)

Moesin Antibody in Immunocytochemistry (ICC/IF)

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Moesin Antibody (MA5-49167) in ICC/IF

Immunocytochemistry analysis of Moesin in SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in Moesin Monoclonal antibody (Product # MA5-49167) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More

Moesin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Moesin Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image Moesin Monoclonal Antibody (8D4)

产品信息

MA5-49167

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

2-5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1-3 µg/10x^6 cells

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

8D4

抗原

E.coli-derived human Moesin/MSN recombinant protein (Position: R184-K568).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_3074825

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human HL-60 whole cell, human K562 whole cell, monkey COS-7 whole cell, rat kidney tissue, mouse kidney tissue, human MCF-7 whole cell. IHC: human gastric adenocarcinoma tissue, human colonic adenocarcinoma tissue, human liver cancer tissue, human placenta tissue. ICC/IF: SiHa cell. Flow: U87 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

Moesin, a member of the talin-4. 1 superfamily, is a linking protein of the submembraneous actin cytoskeleton. It is expressed in macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. It is involved in cell adhesion, migration, and organization of cell surface structures.

仅用于科研。不用于诊断过程。未经明确授权不得转售。