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Invitrogen
Mouse Hematopoietic Lineage Antibody Cocktail, eFluor™ 450, eBioscience™
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Mouse Hematopoietic Lineage Antibody (88-7772-72) in Flow
C57BL/6 bone marrow cells were stained with the Mouse Hematopoietic Lineage eFluor® 450 Cocktail, Anti-Mouse CD117 APC (Product # 17-1171-82) and Anti-Mouse Ly-6A/E (Sca-1) PE (Product # 12-5981-82). Viable cells in the lymphocyte gate were used for analysis. Events displayed on the right plot are gated on lineage negative events, as shown on the left histogram.
产品信息
88-7772-72
产品规格
种属反应
Mouse
已发表种属
C. elegans,
Human,
Mouse
宿主/亚型
Rat
分类
Cocktail
类型
Antibody
偶联物
激发/发射光谱
405/445 nm
查看光谱
形式
Liquid
浓度
20 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The Mouse Hematopoietic Lineage eFluor™ 450 Cocktails contains eFluor™ 450-conjugated antibodies that can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on the expression of cell surface antigens.
This cocktail contains the following antibodies:
Anti-Mouse CD3 (17A2) eFluor™ 450
Anti-Mouse CD45R (B220) (RA3-6B2) eFluor™ 450
Anti-Mouse CD11b (M1/70) eFluor™ 450
Anti-Mouse TER-119 (TER-119) eFluor™ 450
Anti-Mouse Ly-G6 (Gr-1) (RB6-8C5) eFluor™ 450
Applications Reported: The Mouse Hematopoietic Lineage eFluor™ 450 Cocktail has been reported for use in flow cytometric analysis.
Applications Tested: The Mouse Hematopoietic Lineage eFluor™ 450 Cocktail has been pre-titrated and tested by flow cytometric analysis of C57Bl/6 bone marrow cells. This can be used at 20 µL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
靶标信息
Hematopoietic Lineage antibodies can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
