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Mouse anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
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FIGURE: 1 / 4
Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (31464) in WB
Western blot was performed using Mouse anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP (Product # 31464). Whole cell extracts of RD (Lane 1, 2, 3) and SH-SY5Y (Lane 4, 5, 6) treated with 1x PTI for 4 hours were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were transferred onto a nitrocellulose membrane (Product # IB33001) by iBlot™ 3 Western ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
31464
Applications
Tested Dilution
Western Blot (WB)
1:10,000-1:200,000
Immunohistochemistry (IHC)
1:500-1:5,000
Immunocytochemistry (ICC/IF)
1:500-1:5,000
Product Specifications
Species Reactivity
Rabbit
Host/Isotype
Mouse
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
HRP
HRP
HRP
Form
Lyophilized
Concentration
0.8 mg/mL
Amount
800 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.6, with 15mg/mL BSA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG
Cross Adsorption
Against human, goat, mouse and sheep serum proteins
Antibody Form
Whole Antibody
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
This antibody has been successfully used in Western blot, and ICC applications.
Antibody Specificity: This antibody reacts with whole molecule rabbit IgG and with the light chains of other rabbit immunoglobulins. The antibody has minimal inherent cross-reactivity to mouse serum proteins. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, goat, mouse and sheep serum proteins. However, the antibody may cross-react with immunoglobulins from other species.
Restoration and Storage: Store product at 4°C until opened. Restore with 1.0 mL distilled water (0.8 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.
To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Country of Origin: USA
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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