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Invitrogen
Mu-Calpain Monoclonal Antibody (9A4H8D3)
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Mu-Calpain Antibody (MA3-940) in ICC/IF
Immunofluorescent analysis of Mu-Calpain in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Mu-Calpain monoclonal antibody (Product # MA3-940) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Mu-Calpain staining (green), F-Actin... View More
产品信息
MA3-940
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Hamster,
Human,
Mouse,
Pig,
Rabbit,
Rat
已发表种属
Arthropod,
Bovine,
C. elegans,
Fish,
Hamster,
Human,
Mouse,
Pig,
Rabbit,
Rat,
Sheep,
Yeast
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
9A4H8D3
抗原
Purified bovine skeletal muscle 80 kDa mu-calpain subunit.
偶联物
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein A
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA3-940 detects mu-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, mouse lung, pig cultured cells and hamster and rabbit samples. This antibody does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin.
MA3-940 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunocytochemistry procedures. By Western blot, this antibody detects an 80 kDa protein representing mu-calpain from human platelets and erythrocytes and HeLa and A431 cell lysates. Immunocytochemical staining of mu-calpain in LLC-PK1 cells with MA3-940 results in diffuse cytoplasmic staining. This product has not been shown to be effective in immunoprecipitation experiments.
The MA3-940 antigen is purified bovine skeletal muscle 80 kDa mu-calpain subunit. This antibody recognizes an epitope between amino acids 465-520 (domain III) of human mu-calpain.
靶标信息
The calpains including Calpain 1 (CAPN1), calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. Diseases associated with CAPN1 include Spastic Paraplegia 76, Autosomal Recessive and Spasticity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: calcium activated neutral protease; calcium-activated neutral protease 1; Calcium-activated neutral proteinase 1; calcium-dependent protease; calpain 1 large subunit; calpain 1, (mu/I) large subunit; calpain 1, large subunit; Calpain mu-type; calpain, large polypeptide L1; Calpain-1 catalytic subunit; Calpain-1 large subunit; Calpain-I; Calpain-II; CANP 1; Cell proliferation-inducing gene 30 protein; cell proliferation-inducing protein 30; micromolar calcium-activated neutral protease 1; Micromolar-calpain; mu-calpain; mucalpain; muCANP
基因别名: CANP; CANP 1; CANP1; CANPL1; Capa-1; Capa1; CAPN1; Cls1; I79_024883; mu-calpin; muCANP; muCL; PIG30; SPG76
UniProt ID: (Pig) P35750, (Bovine) Q27970, (Human) P07384, (Mouse) O35350, (Rat) P97571
Entrez Gene ID: (Rabbit) 100009089, (Pig) 397027, (Bovine) 281661, (Human) 823, (Mouse) 12333, (Rat) 29153, (Chinese hamster) 100758636
