Invitrogen

Myosin Skeletal Muscle Monoclonal Antibody (414)

Name: Myosin Skeletal Muscle Monoclonal Antibody (414)
Brand: Invitrogen
SKU: MA1-35718

1 篇参考文献

查看其他2个Myosin Skeletal Muscle抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

Myosin Skeletal Muscle Antibody in Western Blot (WB)

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Myosin Skeletal Muscle Antibody (MA1-35718) in WB

Western blot was performed using Myosin Skeletal Muscle Monoclonal Antibody (414) (Product # MA1-35718) and a 223 kDa band corresponding to Myosin Skeletal Muscle was observed in RD, C2C12 differentiated to myotubes and L6 but not in HeLa, A549, MCF7 and PC12. Whole cell extracts (30 µg lysate) of RD (Lane 1), HeLa (Lane 2), A549 (Lane 3), MCF7 (Lane 4), C2C12 (Lane 5), C2C12 differentiated to myotubes (Lan... View More

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Immunogen image

Immunogen Image Myosin Skeletal Muscle Monoclonal Antibody (414)

产品信息

MA1-35718

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:5-1:10

免疫组化（冰冻） (IHC (F))

1:5-1:10

免疫细胞化学 (ICC/IF)

1:10

产品规格

种属反应

Chicken, Human, Mouse, Rabbit, Rat

已发表种属

Rat

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

414

抗原

Chicken skeletal muscle cell preparation

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

tissue culture supernatant

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2147020

产品详细信息

Mab 414 reacts with myosin-containing elements in most cell types. Antigen localization: cytoplasm In Western blot experiments the antibody reacts with a 75kD part of the heavy chain (epitope location on a myosin-common sitechain), no reaction with light chains. It is not reactive with alpha-cardiac myosin. Positive control: muscle, myofibrils (chicken)

靶标信息

Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: 2A myosin heavy chain; 2B myosin heavy chain; epididymis luminal protein 71; fast 2a myosin heavy chain; inclusion body myopathy 3, autosomal dominant; MGC133384; myh4; MyHC-2a; MyHC-2b; MyHC-2x; MyHC-IIa; MyHC-IIb; MyHC-IIx/d; Myosin heavy chain 1; Myosin heavy chain 2; Myosin heavy chain 2a; Myosin heavy chain 2b; Myosin heavy chain 2x; Myosin heavy chain 4; Myosin heavy chain IIa; Myosin heavy chain IIb; Myosin heavy chain IIx/d; myosin heavy chain type IIb; myosin heavy chain type IIx; Myosin heavy chain, skeletal muscle; myosin heavy chain, skeletal muscle, adult; Myosin heavy chain, skeletal muscle, adult 1; Myosin heavy chain, skeletal muscle, adult 2; Myosin heavy chain, skeletal muscle, fetal; Myosin heavy chain, skeletal muscle, juvenile; myosin, heavy chain 1, skeletal muscle, adult; myosin, heavy chain 2, skeletal muscle, adult; myosin, heavy chain 4, skeletal muscle; myosin, heavy polypeptide 1, skeletal muscle, adult; myosin, heavy polypeptide 2, skeletal muscle, adult; myosin, heavy polypeptide 4, skeletal muscle; Myosin-1; Myosin-2; Myosin-4; skeletal muscle myosin heavy chain II-a; type 2X myosin heavy chain; type IIA myosin heavy chain; unnamed protein product

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基因别名: A530084A17Rik; AI506973; HEL71; IBM3; IId; IId/x; MdMs; MHC; MHC-2X/D; MHC2A; MHC2B; MHC2X/D; Minimsc; Minmus; MM; MYH-2B; MYH1; MYH2; MYH2A; MYH2B; MYH4; MYHa; MYHas8; MYHC; MyHC-2A; MyHC-2B; MyHC-2x; MyHC-2X/D; MyHC-IIA; MyHC-IIb; MyHC-IId/x; MYHC-IIX; MyHC-IIx/d; Myhs-f; Myhs-f1; Myhs-f2; MYHSA1; MYHSA2; Myhsf; Myhsf1; Myhsf2; MYPOP