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N-YFP/N-terminal of YFP Polyclonal Antibody
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N-YFP/N-terminal of YFP Antibody (AS11-1776) in WB
Arabidopsis thaliana leaf lysate which is co-expressing test protein 1 with n YFP tags (~60 k Da) and test protein 2 with c YFP-tag (~40 k Da). In each case only one band is visible demonstrating that the antibodies do not cross-react. Sample preparation and immunoblot analysis were carried out as described in Karnik et al., Plant Cell 2015, March 2015 vol. 27 no. 3 675-694. For immunoblot analysis of plant... View More
产品信息
AS11-1776
产品规格
种属反应
Bacteria,
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from N-terminal of YFP protein.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Specific Species Reactivity: N-YFP tagged proteins from Arabidopsis thaliana, Escherichia coli, Nicotiana tabacum
靶标信息
N-YFP is an engineered, non-native coding sequence derived from Aequorea victoria yellow fluorescent protein and used as a genetically encoded reporter tag in living cells; in typical applications it is expressed from plasmid or integrated constructs and localizes according to the fused protein partner, with fluorescence generally detectable throughout the cytosol and nucleus unless the fusion imposes specific targeting. Structurally, N-YFP denotes the N-terminal split fragment of YFP, commonly corresponding to amino acids 1-158, which by itself is non-fluorescent but can reconstitute a functional fluorophore when brought into close proximity with the complementary C-terminal YFP fragment, enabling bimolecular fluorescence complementation readouts. Functionally, N-YFP serves as a protein-fragment complementation reporter to visualize and spatially map protein-protein interactions in vivo and to support live-cell imaging/quantitation workflows; full-length YFP variants used in related constructs (e.g., Venus YFP carrying the A206K monomerizing substitution) are similarly employed as fluorescent fusion partners for noninvasive detection and tracking of recombinant proteins in cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: nYFP; YFP1; YFPN; YN155
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